Controllable assembly of flexible protein nanotubes for loading multifunctional modules
文献类型：期刊论文
| 作者 | Rao, Guibo1,4; Fu, Yan1; Li, Na1,4; Yin, Jiayi1,4; Zhang, Jie1,4; Wang, Manli2,3; Hu, Zhihong2,3; Cao, Sheng1 |
| 刊名 | Acs applied materials & interfaces
 |
| 出版日期 | 2018-08-01 |
| 卷号 | 10期号:30页码:25135-25145 |
| ISSN号 | 1944-8244 |
| 关键词 | Nanotube Self-assembly Helical reconstruction Cryoelectron microscopy Functionality |
| DOI | 10.1021/acsami.8b07611 |
| 通讯作者 | Cao, sheng(caosheng@wh.iov.cn) |
| 英文摘要 | Viruses with filamentous morphologies, such as tobacco mosaic virus (tmv) and m13 bacteriophage, have long been studied as multivalent nanoscaffolds for loading functional motifs. structural assembly of the capsid proteins (cps) of filamentous viruses often requires the presence of dna or rna molecules, which has limited their applications. here, we describe a strategy for controllable assembly of flexible bio-nanotubes consisting of escherichia coli expressed cp of baculovirus helicoverpa armigera nucleopolyhedrovirus (hearnpv) in vitro. these protein-only nanotubes were studied as a new structural platform for high-density presentation of multiple active molecules on the exterior surface by direct fusion of the protein of interest to the n-terminus of hearnpv cp (hacp). structural characterization using cryoelectron microscopy demonstrated that the hacp could assemble into two closely related but structurally distinct tube types, suggesting the tunable hacp interaction network is the major contributor to the flexibility of hacp nanotubes. our flexible nanotubes could tolerate larger molecular modifications compared with tmv-based templates and could be used as promising candidates for versatile molecular loading applications. |
| WOS关键词 | TOBACCO-MOSAIC-VIRUS ; COAT PROTEINS ; DISPLAY ; PARTICLE ; NANOPARTICLES ; CONSTRUCTION ; LUCIFERASE ; RESOLUTION ; SCAFFOLDS ; SUBSTRATE |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000440956000022 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373253 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cao, Sheng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Rao, Guibo,Fu, Yan,Li, Na,et al. Controllable assembly of flexible protein nanotubes for loading multifunctional modules[J]. Acs applied materials & interfaces,2018,10(30):25135-25145. |
| APA | Rao, Guibo.,Fu, Yan.,Li, Na.,Yin, Jiayi.,Zhang, Jie.,...&Cao, Sheng.(2018).Controllable assembly of flexible protein nanotubes for loading multifunctional modules.Acs applied materials & interfaces,10(30),25135-25145. |
| MLA | Rao, Guibo,et al."Controllable assembly of flexible protein nanotubes for loading multifunctional modules".Acs applied materials & interfaces 10.30(2018):25135-25145. |
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来源：武汉病毒研究所
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