Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice
文献类型：期刊论文
| 作者 | Dai, Shiyu; Zhang, Tao; Zhang, Yanfang; Wang, Hualin; Deng, Fei |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 33期号:3页码:213-226 |
| 关键词 | Zikv Baculovirus expression system Virus-like particles (vlps) Neutralizing antibodies |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-018-0030-5 |
| 通讯作者 | Wang, hualin(h.wang@wh.iov.cn) ; Deng, fei(df@wh.iov.cn) |
| 英文摘要 | The newly emerged mosquito-borne zika virus (zikv) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. several zikv vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, dna and rna vaccines. these have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. virus-like particles (vlps) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies. in this study, we used a new strategy to produce zikv vlps based on the baculovirus expression system and demonstrated the feasibility of their use as a vaccine candidate. the pre-membrane (prm) and envelope (e) proteins were co-expressed in insect cells and self-assembled into particles similar to zikv. we found that the zikv vlps could be quickly and easily prepared in large quantities using this system. the vlps were shown to have good immunogenicity in immunized mice, as they stimulated high levels of virus neutralizing antibody titers, zikv-specific igg titers and potent memory t cell responses. thus, the baculovirus-based zikv vlp vaccine is a safe, effective and economical vaccine candidate for use against zikv. |
| WOS关键词 | VACCINE DEVELOPMENT ; PROTEIN ; PROTECTION ; PLATFORMS ; IMMUNITY ; SYSTEMS ; CELLS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000436012100001 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373257 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hualin; Deng, Fei |
| 作者单位 | Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Shiyu,Zhang, Tao,Zhang, Yanfang,et al. Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice[J]. Virologica sinica,2018,33(3):213-226. |
| APA | Dai, Shiyu,Zhang, Tao,Zhang, Yanfang,Wang, Hualin,&Deng, Fei.(2018).Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice.Virologica sinica,33(3),213-226. |
| MLA | Dai, Shiyu,et al."Zika virus baculovirus-expressed virus-like particles induce neutralizing antibodies in mice".Virologica sinica 33.3(2018):213-226. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。