Association of omega with the c-terminal region of the beta' subunit is essential for assembly of rna polymerase in mycobacterium tuberculosis
文献类型：期刊论文
| 作者 | Mao, Chunyou1,2; Zhu, Yan1,2; Lu, Pei1; Feng, Lipeng1,3; Chen, Shiyun1; Hu, Yangbo1 |
| 刊名 | Journal of bacteriology
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 200期号:12页码:9 |
| ISSN号 | 0021-9193 |
| 关键词 | Mycobacterium tuberculosis Transcription Rna polymerase Omega subunit |
| DOI | 10.1128/jb.00159-18 |
| 通讯作者 | Chen, shiyun(sychen@wh.iov.cn) ; Hu, yangbo(ybhu@wh.iov.cn) |
| 英文摘要 | The omega subunit is the smallest subunit of bacterial rna polymerase (rnap). although homologs of omega are essential in both eukaryotes and archaea, this subunit has been known to be dispensable for rnap in escherichia coli and in other bacteria. in this study, we characterized an indispensable role of the omega subunit in mycobacterium tuberculosis. unlike the well-studied e. coli rnap, the m. tuberculosis rnap core enzyme cannot be functionally assembled in the absence of the omega subunit. importantly, substitution of m. tuberculosis omega with omega subunits from e. coli or thermus thermophilus cannot restore the assembly of m. tuberculosis rnap. furthermore, by replacing different regions in m. tuberculosis omega with the corresponding regions from e. coli omega, we found a nonconserved loop region in m. tuberculosis omega essential for its function in rnap assembly. from rnap structures, we noticed that the location of the c-terminal region of the beta' subunit (beta'ctd) in m. tuberculosis rnap but not in e. coli or t. thermophilus rnap is close to the to loop region. deletion of this beta'ctd in m. tuberculosis rnap destabilized the binding of m. tuberculosis omega on rnap and compromised m. tuberculosis core assembly, suggesting that these two regions may function together to play a role in omega-dependent rnap assembly in m. tuberculosis. sequence alignment of the omega loop and the beta'ctd regions suggests that the essential role of omega is probably restricted to mycobacteria. together, our study characterized an essential role of m. tuberculosis omega and highlighted the importance of the omega loop region in m. tuberculosis rnap assembly. importance dna-dependent rna polymerase (rnap), which consists of a multisubumt core enzyme (a(2)alpha beta beta a omega) and a dissociable gamma subunit, is the only enzyme in charge of transcription in bacteria. as the smallest subunit, the roles of to remain omega he least well studied. in escherichia coh and somli other bacteria, the omega subuni is known to be nonessential for rnap. in this study, we revealed an essential role of the omega subunit for rnap assembly in the human pathogen mycobacterium tuberculosis, and a mycobacterium-specific omega loop that plays a role in this function was also characterized. our study provides fresh insights for further characterizing the roles of bacterial omega subunit. |
| WOS关键词 | ESCHERICHIA-COLI ; TRANSCRIPTION INITIATION ; STRUCTURAL BASIS ; PPGPP ; HOLOENZYME ; RPOZ ; PURIFICATION ; ACTIVATION ; EVOLUTION ; STORY |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000432937200010 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373261 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Shiyun; Hu, Yangbo |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Chunyou,Zhu, Yan,Lu, Pei,et al. Association of omega with the c-terminal region of the beta' subunit is essential for assembly of rna polymerase in mycobacterium tuberculosis[J]. Journal of bacteriology,2018,200(12):9. |
| APA | Mao, Chunyou,Zhu, Yan,Lu, Pei,Feng, Lipeng,Chen, Shiyun,&Hu, Yangbo.(2018).Association of omega with the c-terminal region of the beta' subunit is essential for assembly of rna polymerase in mycobacterium tuberculosis.Journal of bacteriology,200(12),9. |
| MLA | Mao, Chunyou,et al."Association of omega with the c-terminal region of the beta' subunit is essential for assembly of rna polymerase in mycobacterium tuberculosis".Journal of bacteriology 200.12(2018):9. |
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来源：武汉病毒研究所
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