Parallel susceptibility testing of bacteria through culture-quantitative pcr in 96-well plates
文献类型：期刊论文
| 作者 | Luo, Jun1,2; Yu, Junping1; Yang, Hang1; Wei, Hongping1 |
| 刊名 | International journal of infectious diseases
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 70页码:86-92 |
| 关键词 | Antibiotic susceptibility testing Thermo-cold lysis Lyophihzed qpcr reagent Quantitative pcr Bacteria |
| ISSN号 | 1201-9712 |
| DOI | 10.1016/j.ijid.2018.03.014 |
| 通讯作者 | Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | Objective: the methods combining culture and quantitative pcr(qpcr) offer new solutions for rapid antibiotic susceptibility testing(ast). however, the multiple steps of dna extraction and cold storage of pcr reagents needed make them unsuitable for rapid high throughput ast. in this study, a parallel culture-qpcr method was developed to overcome above problems. method: in this method, bacteria culture and dna extraction automatically and simultaneously completed through using a common pcr instrument as a controllable heating device. a lyophilized 16s rdna targeted qpcr reagent was also developed, which was stable and could be kept at 4 degrees c for long time and at 37 degrees c for about two months. result: testing of 36 p. aeruginosa isolates and 28 s. aureus isolates showed that the method had good agreements with the standard broth microdilution method, with an overall agreement of 97.22% (95% ci, 85.83-99.51) for p. aeruginosa and 96.43% (95% ci, 79.76-99.81) for s. aureus. this method could test 12 samples against a panel of up to 7 antibiotics simultaneously in two 96-well pcr plates within 4 h, which greatly improves the testing efficiency of the culture-qpcr method. conclusion: with rapidness to obtain results and the capabilities for automation and multiple-sample testing, the parallel culture-qpcr method would have great potentials in clinical labs. (c) 2018 the author(s). published by elsevier ltd on behalf of international society for infectious diseases. |
| WOS关键词 | REAL-TIME PCR ; STAPHYLOCOCCUS-AUREUS ; ANTIMICROBIAL AGENTS ; RESISTANCE ; DIAGNOSTICS |
| WOS研究方向 | Infectious Diseases |
| WOS类目 | Infectious Diseases |
| 语种 | 英语 |
| WOS记录号 | WOS:000432658100016 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373266 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Jun,Yu, Junping,Yang, Hang,et al. Parallel susceptibility testing of bacteria through culture-quantitative pcr in 96-well plates[J]. International journal of infectious diseases,2018,70:86-92. |
| APA | Luo, Jun,Yu, Junping,Yang, Hang,&Wei, Hongping.(2018).Parallel susceptibility testing of bacteria through culture-quantitative pcr in 96-well plates.International journal of infectious diseases,70,86-92. |
| MLA | Luo, Jun,et al."Parallel susceptibility testing of bacteria through culture-quantitative pcr in 96-well plates".International journal of infectious diseases 70(2018):86-92. |
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来源：武汉病毒研究所
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