Isolation and characterization of pectolytic bacterial pathogens infecting potatoes in nakuru county, kenya
文献类型：期刊论文
| 作者 | Muturi, P.1,2; Yu, J.1; Li, J.1; Jiang, M.1; Maina, A. N.3,4; Kariuki, S.5; Mwaura, F. B.4; Wei, H.1,6 |
| 刊名 | Journal of applied microbiology
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 124期号:6页码:1580-1588 |
| 关键词 | 16s rdna Biolog Kenya Pectobacterium carotovorum Potato soft rot Pseudomonas fluorescens |
| ISSN号 | 1364-5072 |
| DOI | 10.1111/jam.13730 |
| 通讯作者 | Wei, h.(hpwei@wh.iov.cn) |
| 英文摘要 | Aims: isolation and characterization of pectolytic bacteria associated with soft rot disease of potatoes in nakuru, kenya, to provide the basis for the development of disease control measures. methods and results: potato tubers showing symptoms of soft rot were collected from different farms in molo and mau narok regions within nakuru county. isolation was done using crystal violet pectate medium (cvpm). out of the 71 isolates that showed growth on cvpm, pathogenicity tests revealed that 36 of them had the ability to macerate tissues of potato tubers. all the isolates yielded a fragment of approximately 1500 bp after 16s rdna amplification. using the biolog microbial identification system, 20 bacterial isolates were identified as pectobacterium carotovorum subsp. carotovorum, 7 were pseudomonas fluorescens b while 9 were ps. fluorescens a. y1/y2 primers successfully amplified pectate lyase-encoding (pel) gene, approximately 434 bp, in all the 20 p. carotovorum species. the virulence of the isolated strains to cause disease, according to pectinolytic tests, varied with change in incubation temperature of the test samples. pectobacterium carotovorum strains were the most virulent at 30 degrees c while disease severity due to infection by ps. fluorescens a strains was high at 20 degrees c compared to the other isolates. conclusion: this study reveals the identity of pectolytic bacterial species from two genera, pectobacterium and pseudomonas, as causative agents of potato soft rot in nakuru, kenya. significance and impact of the study: research findings from this study will aid in developing suitable risk mitigation methods for adoption by farmers to prevent losses due to soft rot. |
| WOS关键词 | SOFT-ROT ; PECTOBACTERIUM-CAROTOVORUM ; PSEUDOMONAS-FLUORESCENS ; ERWINIA-CAROTOVORA ; SP NOV. ; BLACKLEG ; DICKEYA ; ECOLOGY ; TUBERS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000432005600020 |
| 出版者 | WILEY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373267 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, H. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Emerging Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Tech Univ Kenya, Sch Biol & Life Sci, Nairobi, Kenya 4.Univ Nairobi, Sch Biol Sci, Nairobi, Kenya 5.Kenya Govt Med Res Ctr, Ctr Microbiol Res, Nairobi, Kenya 6.Sinoafrica Joint Res Ctr, Nairobi, Kenya |
| 推荐引用方式 GB/T 7714 | Muturi, P.,Yu, J.,Li, J.,et al. Isolation and characterization of pectolytic bacterial pathogens infecting potatoes in nakuru county, kenya[J]. Journal of applied microbiology,2018,124(6):1580-1588. |
| APA | Muturi, P..,Yu, J..,Li, J..,Jiang, M..,Maina, A. N..,...&Wei, H..(2018).Isolation and characterization of pectolytic bacterial pathogens infecting potatoes in nakuru county, kenya.Journal of applied microbiology,124(6),1580-1588. |
| MLA | Muturi, P.,et al."Isolation and characterization of pectolytic bacterial pathogens infecting potatoes in nakuru county, kenya".Journal of applied microbiology 124.6(2018):1580-1588. |
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来源：武汉病毒研究所
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