The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex
文献类型：期刊论文
| 作者 | Wang, Xi1,2; Chen, Cheng1,2; Zhang, Nan1,2; Li, Jiang1; Deng, Fei1; Wang, Hualin1; Vlak, Just M.3; Hu, Zhihong1; Wang, Manli1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 99期号:4页码:585-595 |
| 关键词 | Baculovirus Ac5 Function Pif complex Occlusion body Group i alphabaculovirus |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/jgv.0.001031 |
| 通讯作者 | Hu, zhihong(huzh@wh.iov.cn) ; Wang, manli(wangml@wh.iov.cn) |
| 英文摘要 | Autographa californica nucleopolyhedrovirus (acmnpv) orf5 (ac5) is a group i alphabaculovirus-specific gene of unknown function, although the protein (ac5) was previously reported to be associated with the per os infectivity factor (pif) complex. the purpose of this study was to study the dynamics of ac5 during acmnpv infection and to verify whether it is indeed a component of the pif complex. transcription and expression analyses suggested that ac5 is a late viral gene. an ac5-deleted recombinant acmnpv was generated by homologous recombination. a one-step growth curve assay indicated that ac5 was not required for budded virus (by) production in sf9 cells. scanning electron microscopy and transmission electron microscopy demonstrated that the deletion of ac5 did not affect occlusion body (ob) morphology, and nor did it affect the insertion of occlusion-derived virus (odv) into obs. partially denaturing sds-page and a co-immunoprecipitation assay clearly showed that ac5 was not a component of the pif complex, while the deletion of ac5 did not affect the formation and presence of the pif complex. further analyses showed, however, that ac5 was an ob-specific protein, but it was not detected as a component of bvs or odvs. bioassay experiments showed that the oral infectivity of ac5-deleted acmnpv to third instar spodoptera exigua larvae was not significantly different from that of the ac5-repaired virus. in conclusion, ac5 is an intrinsic protein of obs, instead of being a component of the pif complex, and is not essential for either bv or odv infection. ac5 is awaiting the assignment of another hitherto unknown function. |
| WOS关键词 | PER-OS INFECTIVITY ; CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS ; ENVELOPE FUSION PROTEIN ; INNER NUCLEAR-MEMBRANE ; VIRUS ENVELOPE ; SORTING MOTIF ; BUDDED-VIRUS ; BACULOVIRUS ; GENE ; ENCODES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000431022600016 |
| 出版者 | MICROBIOLOGY SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373269 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Zhihong; Wang, Manli |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol & Joint Lab Invertebrate Viro, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Wageningen Univ & Res, Lab Virol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Wang, Xi,Chen, Cheng,Zhang, Nan,et al. The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex[J]. Journal of general virology,2018,99(4):585-595. |
| APA | Wang, Xi.,Chen, Cheng.,Zhang, Nan.,Li, Jiang.,Deng, Fei.,...&Wang, Manli.(2018).The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex.Journal of general virology,99(4),585-595. |
| MLA | Wang, Xi,et al."The group i alphabaculovirus-specific protein, ac5, is a novel component of the occlusion body but is not associated with odvs or the pif complex".Journal of general virology 99.4(2018):585-595. |
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来源：武汉病毒研究所
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