Improved immune response against hiv-1 env antigen by enhancing eev production via a k151e mutation in the a34r gene of replication-competent vaccinia virus tiantan
文献类型：期刊论文
| 作者 | Yu, Jie1,2; Li, Yaoming1; Zhong, Maohua1; Yang, Jingyi1; Zhou, Dihan1; Zhao, Bali1; Cao, Yuan1; Yan, Hu1; Zhang, Ejuan1; Yang, Yi1 |
| 刊名 | Antiviral research
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 153页码:49-59 |
| 关键词 | Hiv-1 Vaccine Immune response Gene mutation Extracellular enveloped virus (eev) formation |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2018.03.004 |
| 通讯作者 | Zhong, maohua(zmh@wh.iov.cn) ; Yan, huimin(hmyan@wh.iov.cn) |
| 英文摘要 | The development of an effective hiv-1 vaccine is still a global priority. in recent years, vaccinia virus (vv) has been widely used as an hiv-1 vaccine vector, but its immune efficacy against hiv-1 antigens needs to be optimized. the extracellular enveloped virus (eev) of vv is capable of faster entry, earlier release, and long-range dissemination. we hypothesized that an improvement in eev formation by the manipulation of vv genes involved in the eev release would consequently cause an improved expression of the vv carrying hiv-1 env antigen and a subsequent enhanced immune response. to this end, an a34r k151e mutant (rvtt-a34r(mut)) from vv tiantan strain (vtt) with robustly increased eev release was selected to serve as an optimized vaccine vector. the results were consistent with our hypothesis: the a34r mutant-based hiv-1 vaccine candidate rvtt-a3411, ur-env produced more hiv-1 env antigen in vitro and in vivo, and thus led to an improved hiv-1 env specific t cell immune response, binding antibody, and even the neutralizing antibody response in mice without increased virulence. meanwhile, the application of the a34r mutation on another vv-based hiv-1 vaccine candidate, vtkgpe, also exhibited a similar immune enhancement effect with no enhanced virulence. the results in this study suggested that rvtt-a34r(m ut) is a potentially improved vaccine vector candidate for human application. in addition, the improvement of the eev formation via the a34r gene mutation may also be potent in other poxvirus vector-based vaccines against hiv-1 or other pathogens and even cancer in the future. |
| WOS关键词 | EXTRACELLULAR ENVELOPED VIRUS ; PEXA-VEC JX-594 ; HEPATOCELLULAR-CARCINOMA ; POXVIRUS VECTORS ; RHESUS-MONKEYS ; IMMUNOGENICITY ; EFFICACY ; RELEASE ; PROTEIN ; SPREAD |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| WOS类目 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000430893100006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373272 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhong, Maohua; Yan, Huimin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mucosal Immun Res Grp, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Jie,Li, Yaoming,Zhong, Maohua,et al. Improved immune response against hiv-1 env antigen by enhancing eev production via a k151e mutation in the a34r gene of replication-competent vaccinia virus tiantan[J]. Antiviral research,2018,153:49-59. |
| APA | Yu, Jie.,Li, Yaoming.,Zhong, Maohua.,Yang, Jingyi.,Zhou, Dihan.,...&Yan, Huimin.(2018).Improved immune response against hiv-1 env antigen by enhancing eev production via a k151e mutation in the a34r gene of replication-competent vaccinia virus tiantan.Antiviral research,153,49-59. |
| MLA | Yu, Jie,et al."Improved immune response against hiv-1 env antigen by enhancing eev production via a k151e mutation in the a34r gene of replication-competent vaccinia virus tiantan".Antiviral research 153(2018):49-59. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。