Mechanisms and effects on hbv replication of the interaction between hbv core protein and cellular filamin b
文献类型：期刊论文
| 作者 | Li, Yilin1; Sun, Yishuang2; Sun, Fuyun1; Hua, Rong1; Li, Chenlin1; Chen, Lang1; Guo, Deyin1,3; Mu, Jingfang2 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 33期号:2页码:162-172 |
| 关键词 | Filamin b Core Hbv replication |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-018-0023-4 |
| 通讯作者 | Guo, deyin(guodeyin@mail.sysu.edu.cn) ; Mu, jingfang(mujf@wh.iov.cn) |
| 英文摘要 | Hepatitis b virus (hbv) infection is one of the major problems that threatens global health. there have been many studies on hbv, but the relationship between hbv and host factors is largely unexplored and more studies are needed to clarify these interactions. filamin b is an actin-binding protein that acts as a cytoskeleton protein, and it is involved in cell development and several signaling pathways. in this study, we showed that filamin b interacted with hbv core protein, and the interaction promoted hbv replication. the interaction between filamin b and core protein was observed in hek 293t, huh7 and hepg2 cell lines by co-immunoprecipitation and co-localization immnofluoresence. overexpression of filamin b increased the levels of hbv total rnas and pre-genome rna (pgrna), and improved the secretion level of hepatitis b surface antigen (hbsag) and hepatitis b e antigen (hbeag). in contrast, filamin b knockdown inhibited hbv replication, decreased the level of hbv total rnas and pgrna, and reduced the secretion level of hbsag and hbeag. in addition, we found that filamin b and core protein may interact with each other via four blocks of argentine residues at the c-terminus of core protein. in conclusion, we identify filamin b as a novel host factor that can interact with core protein to promote hbv replication in hepatocytes. our study provides new insights into the relationship between hbv and host factors and may provide new strategies for the treatment of hbv infection. |
| WOS关键词 | ACTIN-BINDING PROTEIN ; VIRUS REPLICATION ; LIFE-CYCLE ; NUCLEAR-LOCALIZATION ; MOLECULAR SCAFFOLD ; VIRAL REPLICATION ; CAPSID PROTEIN ; GAG PROTEINS ; FLNB ; MUTATIONS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000431413000006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373273 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Guo, Deyin; Mu, Jingfang |
| 作者单位 | 1.Wuhan Univ, Sch Basic Med Sci, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 3.Sun Yat Sen Univ, Sch Basic Med Shenzhen, Guangzhou 510081, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yilin,Sun, Yishuang,Sun, Fuyun,et al. Mechanisms and effects on hbv replication of the interaction between hbv core protein and cellular filamin b[J]. Virologica sinica,2018,33(2):162-172. |
| APA | Li, Yilin.,Sun, Yishuang.,Sun, Fuyun.,Hua, Rong.,Li, Chenlin.,...&Mu, Jingfang.(2018).Mechanisms and effects on hbv replication of the interaction between hbv core protein and cellular filamin b.Virologica sinica,33(2),162-172. |
| MLA | Li, Yilin,et al."Mechanisms and effects on hbv replication of the interaction between hbv core protein and cellular filamin b".Virologica sinica 33.2(2018):162-172. |
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来源：武汉病毒研究所
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