Simultaneous determination of glucose and sucrose by a dual-working electrode multienzyme sensor flow-injection system
文献类型：期刊论文
| 作者 | ZHANG, XN; RECHNITZ, GA |
| 刊名 | Electroanalysis
 |
| 出版日期 | 1994-05-01 |
| 卷号 | 6期号:5-6页码:361-367 |
| 关键词 | Dual-working electrode multienzyme sensor Glucose Sucrose Simultaneous determination |
| ISSN号 | 1040-0397 |
| 英文摘要 | A dual-working electrode (k, and k2) multienzyme sensor, made of carbon paste, glucose oxidase (god) membrane (on k1), and invertase (inv)/mutarotase (mut)/god membrane (on k2), is proposed for simultaneous determination of glucose and sucrose. interference to the k2 caused by h2o2 produced in the enzyme reaction on k1 can be eliminated by depositing a catalase (cat) layer between k1 and k2. acceleration of glucose mutarotation by mut was found to be three times faster than by phosphate buffer. glucose was determined directly from the response of k1, while sucrose was computed based on a formula derived from a two-point calibration method. the maximum concentration of sucrose that could be determined depended strongly on the concentration of coexisting glucose. the activities of immobilized god, inv, mut, and cat were examined individually and found stable at room temperature for at least 1 month. the final loss of sucrose response activity was found to be due to denaturation of inv. |
| WOS关键词 | ENZYME ELECTRODE ; STARCH |
| WOS研究方向 | Chemistry ; Electrochemistry |
| WOS类目 | Chemistry, Analytical ; Electrochemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:A1994NV67800002 |
| 出版者 | VCH PUBLISHERS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373276 |
| 专题 | 武汉病毒研究所 |
| 作者单位 | 1.UNIV HAWAII,DEPT CHEM,HAWAII BIOSENSOR LAB,HONOLULU,HI 96822 2.CHINESE ACAD SCI,WUHAN INST VIROL,WUHAN 430071,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | ZHANG, XN,RECHNITZ, GA. Simultaneous determination of glucose and sucrose by a dual-working electrode multienzyme sensor flow-injection system[J]. Electroanalysis,1994,6(5-6):361-367. |
| APA | ZHANG, XN,&RECHNITZ, GA.(1994).Simultaneous determination of glucose and sucrose by a dual-working electrode multienzyme sensor flow-injection system.Electroanalysis,6(5-6),361-367. |
| MLA | ZHANG, XN,et al."Simultaneous determination of glucose and sucrose by a dual-working electrode multienzyme sensor flow-injection system".Electroanalysis 6.5-6(1994):361-367. |
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来源：武汉病毒研究所
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