Baculoviruses contain a gene for the large subunit of ribonucleotide reductase
文献类型：期刊论文
| 作者 | vanStrien, EA; Faktor, O; Hu, ZH; Zuidema, D; Goldbach, RW; Vlak, JM |
| 刊名 | Journal of general virology
 |
| 出版日期 | 1997-09-01 |
| 卷号 | 78页码:2365-2377 |
| ISSN号 | 0022-1317 |
| 英文摘要 | In the genomes of two baculoviruses, spodoptera exigua and s. littoralis multicapsid nucleopoly-hedroviruses (semnpv and splimnpv, respectively), an open reading frame (orf) encoding the large subunit of ribonucleotide reductase (rr1) was identified. the predicted amino acid sequences of semnpv and splimnpv rr1 showed high homology to rr1 proteins from eukaryotes (ca. 70% and 80% similarity, respectively), the amino acid residues thought to be involved in catalytic function were conserved in the baculoviral rr1 orfs, the rr1 orfs in semnpv and splimnpv were located in different genomic positions, in semnpv, the rr1 orf was located upstream of the polyhedrin gene, in an anti-genomic orientation, in splimnpv, the rr1 orf preceded the p74 gene. by searching databanks, sequences homologous to the n terminus of rr1 were also detected upstream of the polyhedrin gene of three other baculoviruses, mamestra brassicae multicapsid npv, panolis flammea multicapsid npv and orgyia pseudotsugata single nucleocapsid npv. the baculovirus type species, autographica californica multicapsid npv, however, does not encode rr. a 2.7 kb transcript could be detected throughout infection with semnpv, classifying semnpv rr1 as an early gene, primer extension analysis revealed several early and late start sites, none of the major start sites showed similarity to previously characterized baculoviral transcriptional start motifs, phylogenetic analysis of prokaryotic, eukaryotic and viral rr1 proteins suggested that semnpv and splimnpv acquired the gene for rr1 from a eukaryotic source, but independently from each other. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; VARICELLA-ZOSTER VIRUS ; COMPLETE DNA-SEQUENCE ; EPSTEIN-BARR-VIRUS ; SWINE FEVER VIRUS ; NUCLEOTIDE-SEQUENCE ; VACCINIA VIRUS ; AUTOGRAPHA-CALIFORNICA ; SPODOPTERA-EXIGUA ; TRANSCRIPTIONAL ANALYSIS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997XT71000030 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373297 |
| 专题 | 武汉病毒研究所 |
| 作者单位 | 1.AGR UNIV WAGENINGEN,DEPT VIROL,NL-6709 PD WAGENINGEN,NETHERLANDS 2.HEBREW UNIV JERUSALEM,FAC AGR,DEPT ENTOMOL,IL-76100 REHOVOT,ISRAEL 3.CHINESE ACAD SCI,WUHAN INST VIROL,WUHAN 43071,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | vanStrien, EA,Faktor, O,Hu, ZH,et al. Baculoviruses contain a gene for the large subunit of ribonucleotide reductase[J]. Journal of general virology,1997,78:2365-2377. |
| APA | vanStrien, EA,Faktor, O,Hu, ZH,Zuidema, D,Goldbach, RW,&Vlak, JM.(1997).Baculoviruses contain a gene for the large subunit of ribonucleotide reductase.Journal of general virology,78,2365-2377. |
| MLA | vanStrien, EA,et al."Baculoviruses contain a gene for the large subunit of ribonucleotide reductase".Journal of general virology 78(1997):2365-2377. |
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来源：武汉病毒研究所
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