A novel glycoprotein d-specific monoclonal antibody neutralizes herpes simplex virus
文献类型：期刊论文
| 作者 | Du, Ruikun; Wang, Lili; Xu, Hao; Wang, Zhiying; Zhang, Tao; Wang, Manli; Ning, Yunjia; Deng, Fei; Hu, Zhihong; Wang, Hualin |
| 刊名 | Antiviral research
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 147页码:131-141 |
| 关键词 | Herpes simplex virus Glycoprotein d Monoclonal antibody Neutralization |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2017.10.013 |
| 通讯作者 | Li, yi(liyi@wh.iov.cn) |
| 英文摘要 | The worldwide prevalence of herpes simplex virus (hsv) and the shortage of efficient vaccines and novel therapeutic strategies against hsv are widely global concerns. the abundance on the virion and the major stimulus for the virus-neutralizing antibodies makes gd a predominant candidate for cure of hsv infection. in this study, we generated a monoclonal antibody (mab), termed m27f, targeting to glycoprotein d (gd) of hsv-2, which also has cross-reactivity against hsv-1 gd. it has a high level of neutralizing activity against both hsv-1 and hsv-2, and binds to a highly conserved region (residues 292-297) within the pro-fusion domain of gd. it can effectively block hsv cell-to-cell spread in vitro. the pre-or post-attachment neutralization assay and syncytium formation inhibition assay revealed that m27f neutralizes hsv at the post-binding stage. moreover, therapeutic administration of m27f completely prevented infection-related mortality of mice challenged with a lethal dose of hsv-2. our newly identified epitope for the neutralizing antibody would facilitate studies of gd-based hsv entry or vaccine design, and m27f itself demonstrated a high potential for adaptation as a protective or therapeutic drug against hsv. |
| WOS关键词 | PRO-FUSION DOMAIN ; RECEPTOR-BINDING ; CERVICAL-CANCER ; GENITAL HERPES ; ANTIGENIC SITE ; PROTECT MICE ; INFECTION ; TYPE-2 ; TRANSMISSION ; MECHANISMS |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| WOS类目 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000416394900016 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373306 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, Yi |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Xiaohongshan 44, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Ruikun,Wang, Lili,Xu, Hao,et al. A novel glycoprotein d-specific monoclonal antibody neutralizes herpes simplex virus[J]. Antiviral research,2017,147:131-141. |
| APA | Du, Ruikun.,Wang, Lili.,Xu, Hao.,Wang, Zhiying.,Zhang, Tao.,...&Li, Yi.(2017).A novel glycoprotein d-specific monoclonal antibody neutralizes herpes simplex virus.Antiviral research,147,131-141. |
| MLA | Du, Ruikun,et al."A novel glycoprotein d-specific monoclonal antibody neutralizes herpes simplex virus".Antiviral research 147(2017):131-141. |
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来源：武汉病毒研究所
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