Optimization of enzymatic cell disruption for improving lipid extraction from schizochytrium sp through response surface methodology
文献类型：期刊论文
| 作者 | Lin, Yuanfeng1; Xie, Xinlei1; Yuan, Bo1; Fu, Jie3; Liu, Linyi1; Tian, Hua1; Chen, Tao2; He, Dongping1 |
| 刊名 | Journal of oleo science
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 67期号:2页码:215-224 |
| 关键词 | Schizochytrium sp. Enzymatic cell disruption Response surface methodology Gc-ms |
| ISSN号 | 1345-8957 |
| DOI | 10.5650/jos.ess17166 |
| 通讯作者 | He, dongping(hedp123456@163.com) |
| 英文摘要 | This study is aimed to explore the optimal conditions of cell disruption in the extraction algae oil process, using alkaline protease to disrupt cell of schizochytrium sp. to extract oil in this paper. the effects of enzymatic lysis temperature, enzymatic lysis time, enzyme dosage and ph value on oil yield and dha yield were studied. through the combination of single factor test and response surface design, the optimal cell disruption conditions were screened out. the fatty acid composition of algal oil was analyzed by gas chromatography-massspectrometry (gc-ms). the results showed that when the conditions were: enzymatic lysis temperature 55 degrees c, enzymatic lysis time 9 h, enzyme dosage 3% of biomass and ph 8,oil yield and dha yield reached the highest 14.52 g/l and 7.12 g/l, respectively. when the strains were cultured in 50 l fermentor, oil yield reached 26.27 g/l and dha yield reached 12.89 g/l. they were 1.81 times higher than that in shake-flask cultivation. the optimization experiment provides the basis for the industrial production of schizochytrium sp. |
| WOS关键词 | FISH-OIL ; WALL |
| WOS研究方向 | Chemistry ; Food Science & Technology |
| WOS类目 | Chemistry, Applied ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000424733900010 |
| 出版者 | JAPAN OIL CHEMISTS SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373313 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | He, Dongping |
| 作者单位 | 1.Wuhan Polytech Univ, Coll Food Sci & Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, 44 Xiaohongshan, Wuhan 430071, Hubei, Peoples R China 3.CABIO Bioengn Wuhan Co Ltd, Wuhan 430073, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Yuanfeng,Xie, Xinlei,Yuan, Bo,et al. Optimization of enzymatic cell disruption for improving lipid extraction from schizochytrium sp through response surface methodology[J]. Journal of oleo science,2018,67(2):215-224. |
| APA | Lin, Yuanfeng.,Xie, Xinlei.,Yuan, Bo.,Fu, Jie.,Liu, Linyi.,...&He, Dongping.(2018).Optimization of enzymatic cell disruption for improving lipid extraction from schizochytrium sp through response surface methodology.Journal of oleo science,67(2),215-224. |
| MLA | Lin, Yuanfeng,et al."Optimization of enzymatic cell disruption for improving lipid extraction from schizochytrium sp through response surface methodology".Journal of oleo science 67.2(2018):215-224. |
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来源：武汉病毒研究所
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