Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of zika virus
文献类型：期刊论文
| 作者 | Li, Jia-Qi1,2; Deng, Cheng-Lin1; Gu, Dayong3; Li, Xiao1,2; Shi, Lei3; He, Jian'an3; Zhang, Qiu-Yan1,2; Zhang, Bo1; Ye, Han-Qing1 |
| 刊名 | Antiviral research
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 150页码:148-154 |
| 关键词 | Flavivirus Zika virus Luciferase Antiviral screening Replicon cell line |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2017.12.017 |
| 通讯作者 | Zhang, bo(zhangbo@wh.iov.cn) ; Ye, han-qing(yehq@wh.iov.cn) |
| 英文摘要 | Zika virus (zikv) is an important emerging human pathogen associated with microcephaly, guillain-barre syndrome and meningoencephalitis. developing rapid and reliable hts assay is important for zikv drug discovery. here, we constructed a dicistronic zikv replicon (zikv-pac-rluc-rep) that contained the renilla luciferase (rluc) reporter gene separated from the puromycin n-acetyl-transferase (pac) selectable marker by a short peptide cleavage site. a clonal replicon cell line stably expressing high level of zikv replicon was established by selection with puromycin. by optimizing cell number, compound concentration and incubation time, a robust replicon cell-based hts assay was developed with a calculated z' value of > 0.5. the fully optimized assay was further validated using several known flavivirus replication inhibitors. altogether, the replicon cell-based hts assay developed in this study will facilitate the discovery of antiviral compounds against zikv. |
| WOS关键词 | WEST-NILE-VIRUS ; DRUG DISCOVERY ; DENGUE VIRUS ; INFECTION ; IDENTIFICATION ; REPLICATION ; RNA |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| WOS类目 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000425078700017 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373314 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Bo; Ye, Han-Qing |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shenzhen Acad Inspect & Quarantine, Shenzhen Int Travel Healthcare Ctr, Cent Lab Hlth Quarantine, Guangzhou 518033, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jia-Qi,Deng, Cheng-Lin,Gu, Dayong,et al. Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of zika virus[J]. Antiviral research,2018,150:148-154. |
| APA | Li, Jia-Qi.,Deng, Cheng-Lin.,Gu, Dayong.,Li, Xiao.,Shi, Lei.,...&Ye, Han-Qing.(2018).Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of zika virus.Antiviral research,150,148-154. |
| MLA | Li, Jia-Qi,et al."Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of zika virus".Antiviral research 150(2018):148-154. |
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来源：武汉病毒研究所
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