Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a gram-negative bacterium, cupriavidus sp strain cnp-8
文献类型：期刊论文
| 作者 | Min, Jun1; Wang, Jinpei2; Chen, Weiwei1; Hu, Xiaoke1 |
| 刊名 | Amb express
 |
| 出版日期 | 2018-03-20 |
| 卷号 | 8页码:11 |
| 关键词 | 2-chloro-4-nitrophenol Hydroxyquinol pathway Kinetics Chemotaxis Cupriavidus sp strain cnp-8 |
| ISSN号 | 2191-0855 |
| DOI | 10.1186/s13568-018-0574-7 |
| 通讯作者 | Hu, xiaoke(xkhu@yic.ac.cn) |
| 英文摘要 | Cupriavidus sp. strain cnp-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2c4np) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. it could degrade 2c4np at temperatures from 20 to 40 degrees c and at ph values from 5 to 10, and degrade 2c4np as high as 1.6 mm. kinetics assay showed that biodegradation of 2c4np followed haldane substrate inhibition model, with the maximum specific growth rate (mu(max)) of 0.148/h, half saturation constant (k-s) of 0.022 mm and substrate inhibition constant (k-i) of 0.72 mm. strain cnp-8 was proposed to degrade 2c4np with hydroxyquinol (1,2,4-benzenetriol, bt) as the ring-cleavage substrate. the 2c4np catabolic pathway in strain cnp-8 is significant from those reported in other gram-negative 2c4np utilizers. enzymatic assay indicated that the monooxygenase initiating 2c4np catabolism had different substrates specificity compared with previously reported 2c4np monooxygenations. capillary assays showed that strain cnp-8 exhibited metabolism-dependent chemotactic response toward 2c4np at the optimum concentration of 0.5 mm with a maximum chemotaxis index of 37.5. furthermore, microcosm studies demonstrated that strain cnp-8, especially the pre-induced cells, could remove 2c4np rapidly from the 2c4np-contaminated soil. considering its adaptability to ph and temperature fluctuations and great degradation efficiency against 2c4np, strain cnp-8 could be a promising candidate for the bioremediation of 2c4np-contaminated sites. |
| WOS关键词 | PSEUDOMONAS-MENDOCINA KR1 ; GENE-CLUSTER ; NITROPHENOL MONOOXYGENASE ; CONTAMINATED SOIL ; DEGRADATION ; CATABOLISM ; BIOAUGMENTATION ; KINETICS ; WBC-3 ; 4-MONOOXYGENASE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000427902000002 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373335 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, Xiaoke |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Min, Jun,Wang, Jinpei,Chen, Weiwei,et al. Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a gram-negative bacterium, cupriavidus sp strain cnp-8[J]. Amb express,2018,8:11. |
| APA | Min, Jun,Wang, Jinpei,Chen, Weiwei,&Hu, Xiaoke.(2018).Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a gram-negative bacterium, cupriavidus sp strain cnp-8.Amb express,8,11. |
| MLA | Min, Jun,et al."Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a gram-negative bacterium, cupriavidus sp strain cnp-8".Amb express 8(2018):11. |
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来源：武汉病毒研究所
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