Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots
文献类型：期刊论文
| 作者 | Yin, Wen1,3; Li, Wei1; Li, Qin1,4; Qin, Chong1,3; Zhang, Zhiping1; Zhang, Xiaowei1; Zhang, Xian-En2; Cui, Zongqiang1 |
| 刊名 | Journal of nanoscience and nanotechnology
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 18期号:7页码:4487-4494 |
| 关键词 | Hiv-1 Quantum dot Structure Negative staining Image |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2018.15347 |
| 通讯作者 | Zhang, xian-en() ; Cui, zongqiang() |
| 英文摘要 | Virus internal labeling by inorganic nanoparticle is a suitable technique for single virus imaging. however, it is unknown that the effect of the internal inorganic nanoparticle on morphology and structure of viral core in mature enveloped human immunodeficiency virus 1 (hiv-1). also, the analysis of intact hiv-1 particles and virus-inorganic nanoparticle hybrids is almost impossible with conventional negative staining because hiv-1 is inherently fragile and unstable. herein, we carried out morphological and structural analysis of a newly constructed quantum dot (qd)-encapsulated infectious hiv-1 particle by using an optimized procedure for the electron microscopic analysis of negatively stained hiv-1 particles. the virus fixation and staining conditions were optimized to ensure the integrity of hiv-1, allowing the ready access to structural information on the viral envelope and core. morphological and structural analysis by optimized tem provide key information about viruses, viral core and nanoparticles, which indicates that the encapsulation of quantum dots had no effect on the morphology or mean size of the viral particle, or on the shape, length, width, or angle of the hiv-1 core. |
| WOS关键词 | ELECTRON-MICROSCOPY ; CRYO-EM ; HIV-1 ; MORPHOLOGY ; PARTICLES ; ENVELOPE ; CORES ; PROTEINS ; TRACKING ; VIRIONS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 语种 | 英语 |
| WOS记录号 | WOS:000426057800006 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373339 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En; Cui, Zongqiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Wen,Li, Wei,Li, Qin,et al. Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots[J]. Journal of nanoscience and nanotechnology,2018,18(7):4487-4494. |
| APA | Yin, Wen.,Li, Wei.,Li, Qin.,Qin, Chong.,Zhang, Zhiping.,...&Cui, Zongqiang.(2018).Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots.Journal of nanoscience and nanotechnology,18(7),4487-4494. |
| MLA | Yin, Wen,et al."Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots".Journal of nanoscience and nanotechnology 18.7(2018):4487-4494. |
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来源：武汉病毒研究所
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