Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells
文献类型：期刊论文
| 作者 | Ke, Xianliang1; Zhang, Yuan2; Zheng, Fangliang3; Liu, Yan2; Zheng, Zhenhua2; Xu, Yi1; Wang, Hanzhong2 |
| 刊名 | Chemical communications
 |
| 出版日期 | 2018-02-04 |
| 卷号 | 54期号:10页码:1189-1192 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/c7cc08880a |
| 通讯作者 | Zhang, yuan(zhangyuan@wh.iov.cn) ; Xu, yi(xuyi70@163.com) |
| 英文摘要 | A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a spytag into the viral glycoprotein, followed by specific conjugation with the spycatcher protein on modified quantum dots (qds) through an isopeptide bond. the labelling method is convenient and efficient and shows little attenuation of viral infectivity. therefore, it is a biologically compatible technique for tracking viral infection. |
| WOS关键词 | SINGLE-VIRUS TRACKING ; HOST-CELLS ; METABOLIC INCORPORATION ; ENVELOPED VIRUSES ; SPYTAG/SPYCATCHER ; COMPONENTS ; CHEMISTRY ; MEMBRANE ; PROTEIN |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000423784500007 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373342 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Yuan; Xu, Yi |
| 作者单位 | 1.Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangzhou 510623, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 3.Liaoning Univ, Sch Life Sci, Key Lab Anim Resource & Epidem Dis Prevent, Shenyang 110036, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Xianliang,Zhang, Yuan,Zheng, Fangliang,et al. Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells[J]. Chemical communications,2018,54(10):1189-1192. |
| APA | Ke, Xianliang.,Zhang, Yuan.,Zheng, Fangliang.,Liu, Yan.,Zheng, Zhenhua.,...&Wang, Hanzhong.(2018).Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells.Chemical communications,54(10),1189-1192. |
| MLA | Ke, Xianliang,et al."Spycatcher-spytag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells".Chemical communications 54.10(2018):1189-1192. |
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来源：武汉病毒研究所
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