Productive hbv infection of well-differentiated, hntcp-expressing human hepatoma-derived (huh7) cells
文献类型：期刊论文
| 作者 | Zhou, Ming1,2,3; Zhao, Kaitao1; Yao, Yongxuan1; Yuan, Yifei1; Pei, Rongjuan1; Wang, Yun1; Chen, Jizheng1; Hu, Xue1; Zhou, Yuan1; Chen, Xinwen1 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 32期号:6页码:465-475 |
| 关键词 | Hepatitis b virus (hbv) Na plus /taurocholate cotransporting polypeptide (ntcp) Huh7 Dimethyl sulfoxide (dmso) Polyethylene glycol (peg) Susceptibility |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-017-3983-x |
| 通讯作者 | Wu, chunchen(wucc@wh.iov.cn) |
| 英文摘要 | Feasible and effective cell models for hepatitis b virus (hbv) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. resistance to dimethyl sulfoxide/polyethylene glycol (dmso/peg), hntcp expression, and a differentiated state are the limiting factors for successful hbv infection models. in the present study, we used a hepatoma cell line (huh7d(hntcp)) to overcome these limiting factors so that it exhibits excellent susceptibility to hbv infection. to achieve this goal, different hepatoma cell lines were tested with 2.5% dmso / 4% peg8000, and one resistant cell line (huh7d) was used to construct a stable hntcp-expressing cell line (huh7d(hntcp)) using a recombinant lentivirus system. then, the morphological characteristics and differentiation molecular markers of huh7d(hntcp) cells with or without dmso treatment were characterized. finally, the susceptibility of huh7d(hntcp) cells to hbv infection was assessed. our results showed that huh7d cells were resistant to 2.5% dmso / 4% peg8000, whereas the others were not. huh7d(hntcp) cells were established to express a high level of hntcp compared to liver extracts, and huh7d(hntcp) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under dmso treatment. huh7d(hntcp) cells fully supported the entire lifecycle of hbv infection. this cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines. |
| WOS关键词 | HEPATITIS-B-VIRUS ; PRIMARY TUPAIA HEPATOCYTES ; ADULT HUMAN HEPATOCYTES ; HUMAN FETAL HEPATOCYTES ; C-VIRUS ; REPLICATION ; ENTRY ; LINES ; POLARIZATION ; VECTOR |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419186700003 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373343 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Chunchen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Shenzhen Univ, State & Local Joint Canc Genome Clin Applicat Key, Shenzhen Xenotransplantat Res & Dev Ctr, Shenzhen Peoples Hosp 2,Affiliated Hosp 1, Shenzhen 518035, Peoples R China 3.Sun Yat Sen Univ, Inst Immunol, Zhongshan Sch Med, Guangdong Prov Key Lab Organ Donat & Transplant I, Guangzhou 510080, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Ming,Zhao, Kaitao,Yao, Yongxuan,et al. Productive hbv infection of well-differentiated, hntcp-expressing human hepatoma-derived (huh7) cells[J]. Virologica sinica,2017,32(6):465-475. |
| APA | Zhou, Ming.,Zhao, Kaitao.,Yao, Yongxuan.,Yuan, Yifei.,Pei, Rongjuan.,...&Wu, Chunchen.(2017).Productive hbv infection of well-differentiated, hntcp-expressing human hepatoma-derived (huh7) cells.Virologica sinica,32(6),465-475. |
| MLA | Zhou, Ming,et al."Productive hbv infection of well-differentiated, hntcp-expressing human hepatoma-derived (huh7) cells".Virologica sinica 32.6(2017):465-475. |
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来源：武汉病毒研究所
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