Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt
文献类型：期刊论文
| 作者 | Wei, Cuihua1,2; Liu, Junli3; Maina, Alice Nyambura4,5; Mwaura, Francis B.4; Yu, Junping1; Yan, Chenghui1,2; Zhang, Ruofang3; Wei, Hongping1,6 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 32期号:6页码:476-484 |
| 关键词 | Ralstonia solanacearum Bacterial wilt Potato Bacteriophage therapy |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-017-3987-6 |
| 通讯作者 | Zhang, ruofang(ruofang_zhang@163.com) ; Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. to control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in kenya and china. based on the lytic curves of the phages with the pathogen ralstonia solanacearum, one optimal bacteriophage cocktail, p1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. the preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with r. solanacearum. eighty percent of potato plants could be protected from the bacterial wilt (caused by r. solanacearum reference strain gim1.74 and field isolates), and the p1 cocktail could kill 98% of live bacteria spiked in the sterilized soil at one week after spraying. however, the treatment efficiencies of p1 depended on the timing of application of the phages, the susceptibility of the plants to the bacterial wilt, as well as the virulence of the bacteria infected, suggesting that it is important to apply the phage therapy as soon as possible once there are early signs of the bacterial wilt. these results provide the basis for the development of bacteriophagebased biocontrol of potato bacterial wilt as an alternative to the use of antibiotics. |
| WOS关键词 | RALSTONIA-SOLANACEARUM STRAINS ; PSEUDOMONAS-SOLANACEARUM ; LYTIC BACTERIOPHAGE ; TOMATO |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419186700004 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373344 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Ruofang; Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Emerging Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Inner Mongolia Univ, Inner Mongolia Potato Engn & Technol Res Ctr, Hohhot 010021, Peoples R China 4.Univ Nairobi, Nairobi 00100, Kenya 5.Tech Univ Kenya, Nairobi 00200, Kenya 6.Sino Africa Joint Res Ctr, Nairobi 00200, Kenya |
| 推荐引用方式 GB/T 7714 | Wei, Cuihua,Liu, Junli,Maina, Alice Nyambura,et al. Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt[J]. Virologica sinica,2017,32(6):476-484. |
| APA | Wei, Cuihua.,Liu, Junli.,Maina, Alice Nyambura.,Mwaura, Francis B..,Yu, Junping.,...&Wei, Hongping.(2017).Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt.Virologica sinica,32(6),476-484. |
| MLA | Wei, Cuihua,et al."Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt".Virologica sinica 32.6(2017):476-484. |
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来源：武汉病毒研究所
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