Detection, isolation, and characterization of chikungunya viruses associated with the pakistan outbreak of 2016-2017
文献类型：期刊论文
| 作者 | Liu, Si-Qing1; Li, Xiao1,2; Zhang, Ya-Nan3; Gao, Ai-Li3; Deng, Cheng-Lin1; Li, Jun-Hua1; Jehan, Shoukat4; Jamil, Nadia4; Deng, Fei5; Wei, Hongping1 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 32期号:6页码:511-519 |
| 关键词 | Chikungunya virus (chikv) Pakistan Virus isolation Clinical serum Molecular characterization |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-017-4059-7 |
| 通讯作者 | Zhang, bo(zhangbo@wh.iov.cn) |
| 英文摘要 | The chikungunya virus (chikv) is a mosquito-transmitted alphavirus, which has infected millions of people in africa, asia, americas, and europe since it reemerged in india and indian ocean regions in 2005-2006. starting in the middle of november 2016, chikv has been widely spread, and more than 4,000 cases of infections in humans were confirmed in pakistan. here, we report the first isolation and characterization of chikv from the pakistan outbreak. eight chikv strains were newly isolated from human serum samples using a cell culture procedure. a full-length genome sequence and eight complete envelope (e1) sequences of chikv from pakistan were obtained in this study. alignment of the chikv e1 sequences revealed that the eight new chikv isolates were highly homogeneous, with only two nonsynonymous substitutions found at generally conserved sites (e99 and q235). based on the comparison of 342 e1 sequences, the two nonsynonymous mutations were located in well-recognized domains associated with viral functions such as the cell fusion and vector specificity, suggesting their potential functional importance. phylogenetic analysis indicated that the chikv strains from pakistan originated from chikv circulating in the indian region. this study helps elucidate the epidemics of chikv in pakistan and also provides a foundation for studies of evolution and expansion of chikv in south asia. |
| WOS关键词 | SEMLIKI-FOREST-VIRUS ; CHOLESTEROL DEPENDENCE ; PROTEIN ; EMERGENCE ; EVOLUTION ; MUTATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419186700008 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373345 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Bo |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Ctr Emerging Infect Dis, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100012, Peoples R China 3.Northeast Agr Univ, Sch Life Sci, Harbin 150036, Heilongjiang, Peoples R China 4.Ctr Excellence Sci & Appl Technol, Islamabad 44000, Pakistan 5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Si-Qing,Li, Xiao,Zhang, Ya-Nan,et al. Detection, isolation, and characterization of chikungunya viruses associated with the pakistan outbreak of 2016-2017[J]. Virologica sinica,2017,32(6):511-519. |
| APA | Liu, Si-Qing.,Li, Xiao.,Zhang, Ya-Nan.,Gao, Ai-Li.,Deng, Cheng-Lin.,...&Zhang, Bo.(2017).Detection, isolation, and characterization of chikungunya viruses associated with the pakistan outbreak of 2016-2017.Virologica sinica,32(6),511-519. |
| MLA | Liu, Si-Qing,et al."Detection, isolation, and characterization of chikungunya viruses associated with the pakistan outbreak of 2016-2017".Virologica sinica 32.6(2017):511-519. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。