中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats

文献类型:期刊论文

作者Wang, Yan-Yan1,3,4; Wang, Yong2,3,4; Jiang, Hai-Fei5; Liu, Jun-Hua1,3,4; Jia, Jun2,3,4; Wang, Ke2,3,4; Zhao, Fei5; Luo, Min-Hua5; Luo, Min-Min6; Wang, Xiao-Min1,3,4
刊名Experimental neurology
出版日期2018-02-01
卷号300页码:135-148
关键词Parkinson's disease Motor cortex Subthalamic nucleus Vesicular glutamate transporter Herpes simplex virus tracing
ISSN号0014-4886
DOI10.1016/j.expneurol.2017.11.006
通讯作者Wang, yong(kingwyong@sina.com) ; Wang, xiao-min(xmwang@ccmu.edu.cn)
英文摘要The glutamatergic projection from the motor cortex to the subthalamic nucleus (stn) constitutes the corticobasal ganglia circuit and plays a critical role in the control of movement. emerging evidence shows that the cortico-stn pathway is susceptible to dopamine depletion. specifically in parkinson's disease (pd), abnormal electrophysiological activities were observed in the motor cortex and stn, while the stn serves as a key target of deep brain stimulation for pd therapy. however, direct morphological changes in the cortico-stn connectivity in response to pd progress are poorly understood at present. in the present study, we used a trans synaptic anterograde tracing method with herpes simplex virus-green fluorescent protein (hsv-gfp) to monitor the cortico-stn connectivity in a rat model of pd. we found that the connectivity from the primary motor cortex (m1) to the stn was impaired in parkinsonian rats as manifested by a marked decrease in trans-synaptic infection of hsv-gfp from m1 neurons to stn neurons in unilateral 6-hydroxydopamine (6-ohda)-lesioned rats. ultrastructural analysis with electron microscopy revealed that excitatory synapses in the stn were also impaired in parkinsonian rats. glutamatergic terminals identified by a specific marker (vesicular glutamate transporter 1) were reduced in the stn, while glutamatergic neurons showed an insignificant change in their total number in both the m1 and stn regions. these results indicate that the m1-stn glutamatergic connectivity is downregulated in parkinsonian rats. this downregulation is mediated probably via a mechanism involving the impairments of excitatory terminals and synapses in the stn.
WOS关键词HERPES-SIMPLEX-VIRUS ; DEEP BRAIN-STIMULATION ; DOPAMINE NEURONS ; BASAL GANGLIA ; HYPERDIRECT PATHWAY ; SENSORY CIRCUITS ; DISEASE ; ARCHITECTURE ; DISORDERS ; SYNAPSES
WOS研究方向Neurosciences & Neurology
WOS类目Neurosciences
语种英语
WOS记录号WOS:000422893300014
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
URI标识http://www.irgrid.ac.cn/handle/1471x/2373348
专题武汉病毒研究所
通讯作者Wang, Yong; Wang, Xiao-Min
作者单位1.Capital Med Univ, Dept Neurobiol, Beijing 100069, Peoples R China
2.Capital Med Univ, Dept Physiol, Beijing 100069, Peoples R China
3.Chinese Minist Educ, Key Lab Neurodegenerat Disorders, Beijing 100069, Peoples R China
4.Beijing Inst Brain Disorders, Beijing 100069, Peoples R China
5.Chinese Acad Sci, Wuhan Inst Virol, CAS Ctr Excellence Brain Sci, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
6.Natl Inst Biol Sci, Beijing 102206, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yan-Yan,Wang, Yong,Jiang, Hai-Fei,et al. Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats[J]. Experimental neurology,2018,300:135-148.
APA Wang, Yan-Yan.,Wang, Yong.,Jiang, Hai-Fei.,Liu, Jun-Hua.,Jia, Jun.,...&Wang, Xiao-Min.(2018).Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats.Experimental neurology,300,135-148.
MLA Wang, Yan-Yan,et al."Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats".Experimental neurology 300(2018):135-148.

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来源:武汉病毒研究所

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