Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats
文献类型:期刊论文
作者 | Wang, Yan-Yan1,3,4; Wang, Yong2,3,4; Jiang, Hai-Fei5; Liu, Jun-Hua1,3,4; Jia, Jun2,3,4; Wang, Ke2,3,4; Zhao, Fei5; Luo, Min-Hua5; Luo, Min-Min6; Wang, Xiao-Min1,3,4 |
刊名 | Experimental neurology
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出版日期 | 2018-02-01 |
卷号 | 300页码:135-148 |
关键词 | Parkinson's disease Motor cortex Subthalamic nucleus Vesicular glutamate transporter Herpes simplex virus tracing |
ISSN号 | 0014-4886 |
DOI | 10.1016/j.expneurol.2017.11.006 |
通讯作者 | Wang, yong(kingwyong@sina.com) ; Wang, xiao-min(xmwang@ccmu.edu.cn) |
英文摘要 | The glutamatergic projection from the motor cortex to the subthalamic nucleus (stn) constitutes the corticobasal ganglia circuit and plays a critical role in the control of movement. emerging evidence shows that the cortico-stn pathway is susceptible to dopamine depletion. specifically in parkinson's disease (pd), abnormal electrophysiological activities were observed in the motor cortex and stn, while the stn serves as a key target of deep brain stimulation for pd therapy. however, direct morphological changes in the cortico-stn connectivity in response to pd progress are poorly understood at present. in the present study, we used a trans synaptic anterograde tracing method with herpes simplex virus-green fluorescent protein (hsv-gfp) to monitor the cortico-stn connectivity in a rat model of pd. we found that the connectivity from the primary motor cortex (m1) to the stn was impaired in parkinsonian rats as manifested by a marked decrease in trans-synaptic infection of hsv-gfp from m1 neurons to stn neurons in unilateral 6-hydroxydopamine (6-ohda)-lesioned rats. ultrastructural analysis with electron microscopy revealed that excitatory synapses in the stn were also impaired in parkinsonian rats. glutamatergic terminals identified by a specific marker (vesicular glutamate transporter 1) were reduced in the stn, while glutamatergic neurons showed an insignificant change in their total number in both the m1 and stn regions. these results indicate that the m1-stn glutamatergic connectivity is downregulated in parkinsonian rats. this downregulation is mediated probably via a mechanism involving the impairments of excitatory terminals and synapses in the stn. |
WOS关键词 | HERPES-SIMPLEX-VIRUS ; DEEP BRAIN-STIMULATION ; DOPAMINE NEURONS ; BASAL GANGLIA ; HYPERDIRECT PATHWAY ; SENSORY CIRCUITS ; DISEASE ; ARCHITECTURE ; DISORDERS ; SYNAPSES |
WOS研究方向 | Neurosciences & Neurology |
WOS类目 | Neurosciences |
语种 | 英语 |
WOS记录号 | WOS:000422893300014 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373348 |
专题 | 武汉病毒研究所 |
通讯作者 | Wang, Yong; Wang, Xiao-Min |
作者单位 | 1.Capital Med Univ, Dept Neurobiol, Beijing 100069, Peoples R China 2.Capital Med Univ, Dept Physiol, Beijing 100069, Peoples R China 3.Chinese Minist Educ, Key Lab Neurodegenerat Disorders, Beijing 100069, Peoples R China 4.Beijing Inst Brain Disorders, Beijing 100069, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, CAS Ctr Excellence Brain Sci, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 6.Natl Inst Biol Sci, Beijing 102206, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yan-Yan,Wang, Yong,Jiang, Hai-Fei,et al. Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats[J]. Experimental neurology,2018,300:135-148. |
APA | Wang, Yan-Yan.,Wang, Yong.,Jiang, Hai-Fei.,Liu, Jun-Hua.,Jia, Jun.,...&Wang, Xiao-Min.(2018).Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats.Experimental neurology,300,135-148. |
MLA | Wang, Yan-Yan,et al."Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats".Experimental neurology 300(2018):135-148. |
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来源:武汉病毒研究所
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