Dual-signal readout nanospheres for rapid point-of-care detection of ebola virus glycoprotein
文献类型：期刊论文
| 作者 | Hu, Jiao1,2; Jiang, Yong-Zhong1,2; Wu, Ling-Ling1,2; Wu, Zhen1,2; Bi, Yuhai3,5,6; Wong, Gary5,6; Qiu, Xiangguo7; Chen, Jianjun4,6; Pang, Dai-Wen1,2; Zhang, Zhi-Ling1,2 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2017-12-19 |
| 卷号 | 89期号:24页码:13105-13111 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b02222 |
| 通讯作者 | Chen, jianjun(chenjj@wh.iov.cn) ; Zhang, zhi-ling(zlzhang@whu.edu.cn) |
| 英文摘要 | Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. we developed a sensitive and quantitative lateral flow assay for detection of ebola virus (ebov) glycoprotein with a novel multifunctional nanosphere (rns@au) as a reporter. each rns@au contains hundreds of quantum dots and dozens of au nanoparticles and can achieve enhanced dual signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). antibody (ab) and streptavidin (sa) were simultaneously modified onto the rns@au to label the target and act as signal enhancer. after the target was labeled by the ab rns@au sa and captured on the test line, biotin-modified rns@au was used to amplify the dual signal by the reaction of sa with biotin. the assay enables naked-eye detection of 2 ng/ml glycoprotein within 20 min, and the quantitative detection limit is 0.18 ng/ml. additionally, the assay has been successfully tested in field work for detecting ebov in spiked urine, plasma, and tap water samples and is thus a promising candidate for early diagnosis of suspect infections in ebov-stricken areas. |
| WOS关键词 | FLOW TEST STRIP ; TIME RT-PCR ; MULTIFUNCTIONAL NANOSPHERES ; SENSITIVE DETECTION ; QUANTUM DOTS ; YELLOW-FEVER ; DISEASE ; ASSAY ; FLUORESCENCE ; GUINEA |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000418626300012 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373354 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Jianjun; Zhang, Zhi-Ling |
| 作者单位 | 1.Wuhan Univ, Inst Adv Studies, Key Lab Analyt Chem Biol & Med, Minist Educ,Coll Chem & Mol Sci,State Key Lab Vir, Wuhan 430072, Hubei, Peoples R China 2.Wuhan Univ, Wuhan Inst Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Shenzhen Third Peoples Hosp, State Key Discipline Infect Dis, Shenzhen Key Lab Pathogen & Immun, Shenzhen 518112, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 6.Chinese Acad Sci, Ctr Influenza Res & Early Warning, Beijing 100101, Peoples R China 7.Univ Manitoba, Dept Med Microbiol, Publ Hlth Agcy Canada, Natl Microbiol Lab,Special Pathogens Program, Winnipeg, MB R3E 3R2, Canada |
| 推荐引用方式 GB/T 7714 | Hu, Jiao,Jiang, Yong-Zhong,Wu, Ling-Ling,et al. Dual-signal readout nanospheres for rapid point-of-care detection of ebola virus glycoprotein[J]. Analytical chemistry,2017,89(24):13105-13111. |
| APA | Hu, Jiao.,Jiang, Yong-Zhong.,Wu, Ling-Ling.,Wu, Zhen.,Bi, Yuhai.,...&Zhang, Zhi-Ling.(2017).Dual-signal readout nanospheres for rapid point-of-care detection of ebola virus glycoprotein.Analytical chemistry,89(24),13105-13111. |
| MLA | Hu, Jiao,et al."Dual-signal readout nanospheres for rapid point-of-care detection of ebola virus glycoprotein".Analytical chemistry 89.24(2017):13105-13111. |
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来源：武汉病毒研究所
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