Fungal mannosylation enhances human papillomavirus 16 e7 therapeutic immunity against tc-1 tumors
文献类型：期刊论文
| 作者 | Wang, Zonglin1,2; Wei, Cuihua1,2; Zhang, Yanjun1,2; Wang, Wei1; Zhou, Zheng1; Xiao, Gengfu1 |
| 刊名 | Oncology reports
 |
| 出版日期 | 2018 |
| 卷号 | 39期号:1页码:425-432 |
| 关键词 | Human papillomavirus 16 Mannosylation E7 Therapeutic vaccine Tc-1 tumors |
| ISSN号 | 1021-335X |
| DOI | 10.3892/or.2017.6083 |
| 通讯作者 | Xiao, gengfu(xiaogf@wh.iov.cn) |
| 英文摘要 | Cervical cancer, resulting from infection with human papillomavirus (hpv)16, remains the fourth most common cancer in women worldwide. recently, three prophylactic hpv vaccines targeting high-risk hpvs (particularly hpv16 and hpv18) have been implemented to protect younger women. however, individuals with pre-existing infections have no benefit from prophylactic vaccines. thus, there is an urgent need to develop therapeutic vaccines. hpv16 e7 has been widely utilized as a target for immune therapy of hpv16-associated lesions or cancers, reflecting the sustained existence of this virus in cancerous cells. we developed mannosylated hpv16 e7 (me7) expressed from pichia pastoris as a therapeutic vaccine against hpv16-associated cancer. unmannosylated e7 (e7) was also generated from pichia pastoris as a control. mannosylation enhanced the uptake of me7 by mannose receptors of bone marrow-derived dendritic cells (bmdcs), while the uptake of e7 was unaffected. me7-uptake bmdcs in vitro induced more ifn-gamma secretion by splenocytes of immunized mice than e7. vaccination of c57bl/6 mice with me7 combined with adjuvant monophosphoryl lipid a (mpl) elicited stronger th1 (type 1 t helper cell) responses and e7-specific t cell responses than e7. the me7 vaccine induced the increased production of ifn-gamma, il-2 and tnf-alpha, elicited more e7-specific ifn-gamma-secreting cd8(+) t cells in spleen and peripheral blood mononuclear cells (pmbcs) and promoted stronger e7-specific cytotoxic cd8(+) t cell responses compared with e7. furthermore, tc-1 tumor challenged mice were used to confirm the antitumor activity of the vaccines. as a result, me7 generated complete antitumor activity against tc-1 tumors, while e7 only provided partial antitumor activity. taken together, me7 can be a promising immunotherapy for treating cervical cancer. |
| WOS关键词 | DENDRITIC CELLS ; MANNOSE RECEPTOR ; DNA VACCINATION ; CERVICAL-CANCER ; ANTIGEN ; IMMUNOGENICITY ; VACCINES ; MICE ; CYTOKINES ; RESPONSES |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000418358200046 |
| 出版者 | SPANDIDOS PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373356 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xiao, Gengfu |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zonglin,Wei, Cuihua,Zhang, Yanjun,et al. Fungal mannosylation enhances human papillomavirus 16 e7 therapeutic immunity against tc-1 tumors[J]. Oncology reports,2018,39(1):425-432. |
| APA | Wang, Zonglin,Wei, Cuihua,Zhang, Yanjun,Wang, Wei,Zhou, Zheng,&Xiao, Gengfu.(2018).Fungal mannosylation enhances human papillomavirus 16 e7 therapeutic immunity against tc-1 tumors.Oncology reports,39(1),425-432. |
| MLA | Wang, Zonglin,et al."Fungal mannosylation enhances human papillomavirus 16 e7 therapeutic immunity against tc-1 tumors".Oncology reports 39.1(2018):425-432. |
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来源：武汉病毒研究所
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