Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis
文献类型：期刊论文
| 作者 | Zhang, Z. -T.1,2; Wang, D. -B.2; Li, C. -Y.3; Deng, J. -Y.4; Zhang, J. -B.1; Bi, L. -J.2; Zhang, X. -E.2 |
| 刊名 | Journal of applied microbiology
 |
| 出版日期 | 2018 |
| 卷号 | 124期号:1页码:286-293 |
| 关键词 | Drug resistance Drug susceptibility testing Microbial sensor Mycobacterium tuberculosis Oxygen electrode |
| ISSN号 | 1364-5072 |
| DOI | 10.1111/jam.13617 |
| 通讯作者 | Zhang, x. -e.(zhangxe@ibp.ac.cn) |
| 英文摘要 | Aimsdrug susceptibility testing (dst) of clinical isolates of mycobacterium tuberculosis is critical in treating tuberculosis. we demonstrate the possibility of using a microbial sensor to perform dst of m. tuberculosis and shorten the time required for dst. methods and resultsthe sensor is made of an oxygen electrode with m. tuberculosis cells attached to its surface. this sensor monitors the residual oxygen consumption of m. tuberculosis cells after treatment with anti-tb drugs with glycerine as a carbon source. in principle, after drug pretreatment for 4-5days, the response differences between the sensors made of drug-sensitive isolates are distinguishable from the sensors made of drug-resistant isolates. the susceptibility of the m. tuberculosis h37ra strain, its mutants and 35 clinical isolates to six common anti-tb drugs: rifampicin, isoniazid, streptomycin, ethambutol, levofloxacin and para-aminosalicylic acid were tested using the proposed method. the results agreed well with the gold standard method (lj) and were determined in significantly less time. the whole procedure takes approximately 11days and therefore has the potential to inform clinical decisions. conclusionsto our knowledge, this is the first study that demonstrates the possible application of a dissolved oxygen electrode-based microbial sensor in m. tuberculosis drug resistance testing. this study used the microbial sensor to perform dst of m. tuberculosis and shorten the time required for dst. significance and impact of the studythe overall detection result of the microbial sensor agreed well with that of the conventional lj proportion method and takes less time than the existing phenotypic methods. in future studies, we will build an o-2 electrode array microbial sensor reactor to enable a high-throughput drug resistance analysis. |
| WOS关键词 | POINT-OF-CARE ; MTBDRPLUS ASSAY ; RESISTANCE ; RIFAMPIN ; TECHNOLOGIES ; DIAGNOSTICS ; ACID |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000418453800026 |
| 出版者 | WILEY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373357 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, X. -E. |
| 作者单位 | 1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agromicrobiol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing, Peoples R China 3.Capital Med Univ, Beijing Chest Hosp, Beijing, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Z. -T.,Wang, D. -B.,Li, C. -Y.,et al. Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis[J]. Journal of applied microbiology,2018,124(1):286-293. |
| APA | Zhang, Z. -T..,Wang, D. -B..,Li, C. -Y..,Deng, J. -Y..,Zhang, J. -B..,...&Zhang, X. -E..(2018).Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis.Journal of applied microbiology,124(1),286-293. |
| MLA | Zhang, Z. -T.,et al."Microbial sensor for drug susceptibility testing of mycobacterium tuberculosis".Journal of applied microbiology 124.1(2018):286-293. |
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来源：武汉病毒研究所
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