Protein inhibitor of activated stat2 restricts hcv replication by modulating viral proteins degradation
文献类型：期刊论文
| 作者 | Guo, Jing1,2; Chen, Dan1,2; Gao, Xiaoxiao1,2; Hu, Xue1; Zhou, Yuan1; Wu, Chunchen1; Wang, Yun1; Chen, Jizheng1; Pei, Rongjuan1; Chen, Xinwen1 |
| 刊名 | Viruses-basel
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 9期号:10页码:15 |
| 关键词 | Hepatitis c virus Pias2 Protein stability |
| ISSN号 | 1999-4915 |
| DOI | 10.3390/v9100285 |
| 通讯作者 | Pei, rongjuan(rongjuan_pei@wh.iov.cn) ; Chen, xinwen(chenxw@wh.iov.cn) |
| 英文摘要 | Hepatitis c virus (hcv) replication in cells is controlled by many host factors. in this report, we found that protein inhibitor of activated stat2 (pias2), which is a small ubiquitin-like modifier (sumo) e3 ligase, restricted hcv replication. during infection, hcv core, ns3 and ns5a protein expression, as well as the viral assembly and budding efficiency were enhanced when endogenous pias2 was knocked down, whereas exogenous pias2 expression decreased hcv core, ns3, and ns5a protein expression and the viral assembly and budding efficiency. pias2 did not influence the viral entry, rna replication, and protein translation steps of the viral life cycle. when expressed together with sumo1, pias2 reduced the hcv core, ns3 and ns5a protein levels expressed from individual plasmids through the proteasome pathway in a ubiquitin-independent manner; the stability of these proteins in the hcv infectious system was enhanced when pias2 was knocked down. furthermore, we found that the core was sumoylated at amino acid k78, and pias2 enhanced the sumoylation level of the core. |
| WOS关键词 | HEPATITIS-C VIRUS ; VESICULAR STOMATITIS-VIRUS ; LIPID DROPLET ; CELL-CULTURE ; SUMOYLATION ; INFECTION ; SUMO ; EXPRESSION ; UBIQUITIN ; COMPLEX |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000414617500022 |
| 出版者 | MDPI AG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373363 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Pei, Rongjuan; Chen, Xinwen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Jing,Chen, Dan,Gao, Xiaoxiao,et al. Protein inhibitor of activated stat2 restricts hcv replication by modulating viral proteins degradation[J]. Viruses-basel,2017,9(10):15. |
| APA | Guo, Jing.,Chen, Dan.,Gao, Xiaoxiao.,Hu, Xue.,Zhou, Yuan.,...&Chen, Xinwen.(2017).Protein inhibitor of activated stat2 restricts hcv replication by modulating viral proteins degradation.Viruses-basel,9(10),15. |
| MLA | Guo, Jing,et al."Protein inhibitor of activated stat2 restricts hcv replication by modulating viral proteins degradation".Viruses-basel 9.10(2017):15. |
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来源：武汉病毒研究所
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