Targeted delivery of sirna using rgdfc-conjugated functionalized selenium nanoparticles for anticancer therapy
文献类型：期刊论文
| 作者 | Xia, Yu1; Lin, Zhengfang1; Li, Yinghua1; Zhao, Mingqi1; Wang, Changbing1; Guo, Min1; Zhang, Bo2; Zhu, Bing1 |
| 刊名 | Journal of materials chemistry b
 |
| 出版日期 | 2017-09-07 |
| 卷号 | 5期号:33页码:6941-6952 |
| ISSN号 | 2050-750X |
| DOI | 10.1039/c7tb01315a |
| 通讯作者 | Zhang, bo(zhangbowh2017@hotmail.com) ; Zhu, bing(zhubing2017@hotmail.com) |
| 英文摘要 | Lack of biocompatible and effective delivery carriers is a significant shortcoming for sirna-mediated cancer therapy. to overcome these limitations, selenium nanoparticles (senps) have been proposed for sirna transfection vehicles. in this study, we synthesized novel rgdfc peptide modified selenium nanoparticles (rgdfc-senps) as a gene vehicle, which was expected to improve the tumor-targeted delivery activity. rgdfc-senps were compacted with sirnas (anti-oct4) by electrostatic interaction, which was capable of protecting sirna from degradation. rgdfc-senps exhibited excellent ability to deliver sirna into hepg2 cells. sirna transfection assay showed that rgdfc-senps presented a higher gene silencing efficacy than conventional lipofectamine 2000. the cytotoxicity of rgdfc-senps/sirna on normal cells was lower than that on tumor cells, indicating that rgdfc-senps/sirna exhibited selectivity between normal and cancer cells. additionally, oct4 knockdown mediated by the selenium nanoparticle transfection arrested hepg2 cells mainly at the g2/m phase and significantly induced hepg2 cell apoptosis. western blotting results showed that rgdfc-senps/sirna might trigger wnt/beta-catenin signaling, and further activate a bcl-2 apoptosis-related signaling pathway to advance hepg2 cell apoptosis. in vivo biodistribution experiments indicated that rgdfc-senps/sirna nanoparticles were specifically targeted to the hepg2 tumors. most importantly, rgdfc-senps/sirna inhibited tumor growth significantly and induced hepg2 cell apoptosis via silencing the oct4 gene. in addition, the results of h&e staining demonstrated that rgdfc-senps/sirna had negligible toxicity on the major organs of mice. in a word, this study provides a novel strategy for the design of biocompatible and effective sirna delivery vehicles in cancer therapy. |
| WOS关键词 | MESOPOROUS SILICA NANOPARTICLES ; CANCER STEM-CELLS ; CALCIUM-PHOSPHATE NANOPARTICLES ; IN-VIVO ; MULTIDRUG-RESISTANCE ; ANTITUMOR EFFICACY ; POOLED SIRNAS ; LIVER-CANCER ; CO-DELIVERY ; EFFICIENT |
| WOS研究方向 | Materials Science |
| WOS类目 | Materials Science, Biomaterials |
| 语种 | 英语 |
| WOS记录号 | WOS:000408270200023 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373381 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Bo; Zhu, Bing |
| 作者单位 | 1.Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Cent Lab, Guangzhou 510120, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Yu,Lin, Zhengfang,Li, Yinghua,et al. Targeted delivery of sirna using rgdfc-conjugated functionalized selenium nanoparticles for anticancer therapy[J]. Journal of materials chemistry b,2017,5(33):6941-6952. |
| APA | Xia, Yu.,Lin, Zhengfang.,Li, Yinghua.,Zhao, Mingqi.,Wang, Changbing.,...&Zhu, Bing.(2017).Targeted delivery of sirna using rgdfc-conjugated functionalized selenium nanoparticles for anticancer therapy.Journal of materials chemistry b,5(33),6941-6952. |
| MLA | Xia, Yu,et al."Targeted delivery of sirna using rgdfc-conjugated functionalized selenium nanoparticles for anticancer therapy".Journal of materials chemistry b 5.33(2017):6941-6952. |
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来源：武汉病毒研究所
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