Development of a luciferase-based biosensor to assess enterovirus 71 3c protease activity in living cells
文献类型：期刊论文
| 作者 | Zhang, Yuan1; Ke, Xianliang2; Zheng, Caishang2; Liu, Yan1; Xie, Li1; Zheng, Zhenhua1; Wang, Hanzhong1 |
| 刊名 | Scientific reports
 |
| 出版日期 | 2017-09-04 |
| 卷号 | 7页码:11 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-10840-x |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Enterovirus 71 (ev71) is a major pathogen of hand, foot, and mouth disease (hfmd). to date, no antiviral drug has been approved to treat ev71 infection. due to the essential role that ev71 3 c protease (3c(pro)) plays in the viral life cycle, it is generally considered as a highly appealing target for antiviral drug development. in this study, we present a transgene-encoded biosensor that can accurately, sensitively and quantitatively report the proteolytic activity of ev71 3c(pro). this biosensor is based on the catalyzed activity of a pro-interleukin (il)-1 beta-enterovirus 3c(pro) cleavage sitegaussia luciferase (gluc) fusion protein that we named i-3cs-gluc. gluc enzyme is inactive in the fusion protein because of aggregation caused by pro-il-1 beta. however, the 3cpro of ev71 and other enteroviruses, such as coxsackievirus a9 (cva9), coxsackievirus b3 (cvb3), and poliovirus can recognize and process the canonical enterovirus 3c(pro) cleavage site between pro-il-1 beta and gluc, thereby releasing and activating gluc and resulting in increased luciferase activity. the high sensitivity, ease of use, and applicability as a transgene in cell-based assays of i-3cs-gluc biosensor make it a powerful tool for studying viral protease proteolytic events in living cells and for achieving high-throughput screening of antiviral agents. |
| WOS关键词 | IN-VIVO ; FUNCTION PREDICTION ; FIREFLY LUCIFERASE ; GAUSSIA LUCIFERASE ; MOUTH-DISEASE ; INFECTION ; CHILDREN ; BIOLUMINESCENCE ; RUPINTRIVIR ; EXPRESSION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000408997700084 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373383 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Guangzhou Women & Children Med Ctr, Guangzhou Inst Pediat, Guangzhou 510623, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuan,Ke, Xianliang,Zheng, Caishang,et al. Development of a luciferase-based biosensor to assess enterovirus 71 3c protease activity in living cells[J]. Scientific reports,2017,7:11. |
| APA | Zhang, Yuan.,Ke, Xianliang.,Zheng, Caishang.,Liu, Yan.,Xie, Li.,...&Wang, Hanzhong.(2017).Development of a luciferase-based biosensor to assess enterovirus 71 3c protease activity in living cells.Scientific reports,7,11. |
| MLA | Zhang, Yuan,et al."Development of a luciferase-based biosensor to assess enterovirus 71 3c protease activity in living cells".Scientific reports 7(2017):11. |
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来源：武汉病毒研究所
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