Molecular cloning and characterization of the genes encoding the proteins of zika virus
文献类型：期刊论文
| 作者 | Hou, Wangheng1; Cruz-cosme, Ruth1; Armstrong, Najealicka1; Obwolo, Lilian Akello1; Wen, Fayuan1; Hu, Wenhui2; Luo, Min-Hua3; Tang, Qiyi1 |
| 刊名 | Gene
 |
| 出版日期 | 2017-09-10 |
| 卷号 | 628页码:117-128 |
| 关键词 | Zika virus (zikv) Capsid Envelope Nonstructural (ns) proteins Centrosome Mitochondria Endoplasmic reticulum (er) Golgi apparatus Endosome Autophagy |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2017.07.049 |
| 通讯作者 | Tang, qiyi(qiyi.tang@howard.edu) |
| 英文摘要 | Zika virus (zikv) encodes a precursor protein (also called poiyprotein) of about 3424 amino acids that is processed by proteases to generate 10 mature proteins and a small peptide. in the present study, we characterized the chemical features, suborganelle distribution and potential function of each protein using flag-tagged protein expression system. western blot analysis revealed the molecular weight of the proteins and the polymerization of e, ns1, and ns3 proteins. in addition, we performed multi-labeled fluorescent immunocytochemistry and subcellular fractionation to determine the subcellular localization of these proteins in host cells. we found that 1) the capsid protein colocalizes with 3 different cellular organelles: nucleoli, golgi apparatus, and lipid droplet; ns2b and ns4a are associated with the golgi apparatus; 2) the capsid and ns1proteins distribute in both cytoplasm and nucleus, ns5 is a nuclear protein; 3) ns3 protein colocalizes with tubulin and affects lamin a; 4) envelope, prm, and ns2a proteins co-localize with the endoplasmic reticulum; 5) ns1 is associated with autophagosomes and ns4b is related to early endosome; 6) ns5 forms punctate structures in the nucleus that associate with splicing compartments shown by sc35, leading to reduction of sc35 protein level and trafficking of sc35 from the nucleus to the cytoplasm. these data suggest that zikv generates 10 functional viral proteins that exhibit distinctive subcellular distribution in host cells. |
| WOS关键词 | 3 SPLICE-SITE ; IN-VITRO ; BRAIN ORGANOIDS ; CELL-CYCLE ; EVOLUTION ; PERSPECTIVES ; REPLICATION ; ISOLATIONS ; EMERGENCE ; HISTORY |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000412256800016 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373388 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Tang, Qiyi |
| 作者单位 | 1.Howard Univ, Coll Med, Dept Microbiol, Seeley Mudd Bldg,520 W St NW, Washington, DC 20059 USA 2.Temple Univ, Lewis Katz Sch Med, Dept Pathol & Lab Med, Ctr Metab Dis Res, 3500 N Broad St, Philadelphia, PA 19140 USA 3.Chinese Acad Sci, Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Wangheng,Cruz-cosme, Ruth,Armstrong, Najealicka,et al. Molecular cloning and characterization of the genes encoding the proteins of zika virus[J]. Gene,2017,628:117-128. |
| APA | Hou, Wangheng.,Cruz-cosme, Ruth.,Armstrong, Najealicka.,Obwolo, Lilian Akello.,Wen, Fayuan.,...&Tang, Qiyi.(2017).Molecular cloning and characterization of the genes encoding the proteins of zika virus.Gene,628,117-128. |
| MLA | Hou, Wangheng,et al."Molecular cloning and characterization of the genes encoding the proteins of zika virus".Gene 628(2017):117-128. |
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来源：武汉病毒研究所
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