Isolation and characterization of a novel mesonivirus from culex mosquitoes in china
文献类型：期刊论文
| 作者 | Wang, Yujuan1,2; Xia, Han1; Zhang, Bo1; Liu, Xiaoyun1,2; Yuan, Zhiming1 |
| 刊名 | Virus research
 |
| 出版日期 | 2017-08-15 |
| 卷号 | 240页码:130-139 |
| 关键词 | Nidoviruses Mesoniviridae Yichang virus Phylogeny Taxonomic |
| ISSN号 | 0168-1702 |
| DOI | 10.1016/j.virusres.2017.08.001 |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) |
| 英文摘要 | A new insect nidovirus (named yichang virus) from the family mesoniviridae was isolated, identified, and characterized from culex mosquitoes in hubei, china. results showed a high number of viral rna copies (up to 10(11) copies/ml) within 48 h in c6/36 cells. in addition, the titers of the yichang virus reached maximal levels of 10(7) pfu/ml at 6 d post-infection (dpi). the virus produced moderate cytopathic effects when the multiplicity of infection ranged from 0.001-0.1 at 6 dpi, but did not replicate in mammalian cells. under electron microscopy, the virion of the yichang virus appeared as spherical particles with diameters of similar to 80 nm and large club-shaped projections. although subsequent genomic sequence analysis revealed that the yichang virus had similar protein patterns as those of other mesoniviruses, the nucleotide acids shared less than 20% blast query coverage with known viruses in the family mesoniviridae, and showed a maximum sequence identity of 67% for rna-dependent rna polymerase (rdrp). the putative protein sequences showed slightly higher identity (28%-68%), and the most conserved domain was rdrp. based on the phylogenetic and pairwise evolutionary distance analyses, the yichang virus should be considered a new species belonging to a currently unassigned genus within the family mesoniviridae. |
| WOS关键词 | SARS-CORONAVIRUS ; 3C-LIKE PROTEASE ; VIRUS ; PREDICTION ; EXORIBONUCLEASE ; DISCOVERY ; SEQUENCE ; SERVER ; SITES |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000413133000017 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373399 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yujuan,Xia, Han,Zhang, Bo,et al. Isolation and characterization of a novel mesonivirus from culex mosquitoes in china[J]. Virus research,2017,240:130-139. |
| APA | Wang, Yujuan,Xia, Han,Zhang, Bo,Liu, Xiaoyun,&Yuan, Zhiming.(2017).Isolation and characterization of a novel mesonivirus from culex mosquitoes in china.Virus research,240,130-139. |
| MLA | Wang, Yujuan,et al."Isolation and characterization of a novel mesonivirus from culex mosquitoes in china".Virus research 240(2017):130-139. |
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来源：武汉病毒研究所
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