Frontline science: nasal epithelial gm-csf contributes to tlr5-mediated modulation of airway dendritic cells and subsequent iga response
文献类型：期刊论文
| 作者 | Cao, Yuan; Zhang, Ejuan; Yang, Jingyi; Yang, Yi; Yu, Jie; Xiao, Yang; Li, Wei; Zhou, Dihan; Li, Yaoming; Zhao, Bali |
| 刊名 | Journal of leukocyte biology
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 102期号:3页码:575-587 |
| 关键词 | Innate immunity Adaptive immunity Cell activation Adjuvant |
| ISSN号 | 0741-5400 |
| DOI | 10.1189/jlb.3hi0816-368rr |
| 通讯作者 | Zhong, maohua(zmh@wh.iov.cn) ; Yan, huimin(hmyan@wh.iov.cn) |
| 英文摘要 | Flagellin, as a tlr5 agonist, is an established mucosal adjuvant for enhancing mucosal iga responses by i.n. immunization. nasal epithelial cells (necs) are the first sentinel cells to be exposed to antigen and adjuvant in i.n. immunization, and it is suggested that they play an important role in the mucosal adjuvant activity of flagellin. however, the molecular mechanism leading to modulation and the response by flagellin-activated necs remain unknown. we aimed to identify the soluble mediator(s) from flagellin-activated necs that modulate the functions of airway dendritic cells (dcs) and enhance subsequent iga response. in vitro studies showed that compared with the tlr4 agonist lps, flagellin directly triggered slight up-regulation of cd80 on airway dcs but was insufficient to affect cd86 expression and dc-mediated iga response. with the use of an in vitro system for culturing mouse primary necs (mnecs), we demonstrated that flagellin-activated mnecs could functionally modulate airway dcs, which manifested as significant up-regulation of cd80/cd86 and enhancement of iga production. the functional modulation of airway dcs was dependent on tlr5 activation of mnecs rather than direct tlr5 activation of airway dcs. with the use of cytokine array and antibody-blocking assays, we further identified that gm-csf, a cytokine secreted from tlr5-activated mnecs, contributes to the activation of mnecs to airway dcs and subsequent iga enhancement. in vivo blocking experiments confirmed that gm-csf is an important factor in recombinant flagellin derived from salmonella typhi (flic)-induced airway dc activation and antigen-specific iga enhancement. our data directly demonstrate that nasal epithelial gm-csf contributes to tlr5-mediated modulation of airway dcs and a subsequent iga response. |
| WOS关键词 | COLONY-STIMULATING FACTOR ; MUCOSAL ADJUVANT ACTIVITY ; INFLUENZA VACCINE ; HUMORAL IMMUNITY ; INDUCE IGA ; FLAGELLIN ; RECEPTOR ; EXPRESSION ; PROMOTES ; AGONIST |
| WOS研究方向 | Cell Biology ; Hematology ; Immunology |
| WOS类目 | Cell Biology ; Hematology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000413395700005 |
| 出版者 | FEDERATION AMER SOC EXP BIOL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373401 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhong, Maohua; Yan, Huimin |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Mucosal Immun Res Grp, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Yuan,Zhang, Ejuan,Yang, Jingyi,et al. Frontline science: nasal epithelial gm-csf contributes to tlr5-mediated modulation of airway dendritic cells and subsequent iga response[J]. Journal of leukocyte biology,2017,102(3):575-587. |
| APA | Cao, Yuan.,Zhang, Ejuan.,Yang, Jingyi.,Yang, Yi.,Yu, Jie.,...&Yan, Huimin.(2017).Frontline science: nasal epithelial gm-csf contributes to tlr5-mediated modulation of airway dendritic cells and subsequent iga response.Journal of leukocyte biology,102(3),575-587. |
| MLA | Cao, Yuan,et al."Frontline science: nasal epithelial gm-csf contributes to tlr5-mediated modulation of airway dendritic cells and subsequent iga response".Journal of leukocyte biology 102.3(2017):575-587. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。