Construction and rescue of a functional synthetic baculovirus
文献类型：期刊论文
| 作者 | Shang, Yu1,2; Wang, Manli1; Xiao, Gengfu1; Wang, Xi1,2; Hou, Dianhai1; Pan, Kai1,2; Liu, Shurui1,2; Li, Jiang1; Wang, Jun1; Arif, Basil M.3 |
| 刊名 | Acs synthetic biology
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 6期号:7页码:1393-1402 |
| 关键词 | Synthetic genome Baculovirus Acmnpv Large dna virus |
| ISSN号 | 2161-5063 |
| DOI | 10.1021/acssynbio.7b00028 |
| 通讯作者 | Deng, fei(df@wh.iov.cn) ; Hu, zhihong(huzh@wh.iov.cn) |
| 英文摘要 | Synthetic viruses provide a powerful platform to delve deeper into the nature and function of viruses as well as to engineer viruses with novel properties. so far, most synthetic viruses have been rna viruses (<30 kb) and small dna viruses, such as bacteriophage phix174. baculoviruses contain a large circular dsdna genome of 80-180 kb and have been used as biocontrol agents and protein expression vectors. here, we report on the first synthesis of a baculovirus based on the type species autographa californica nucleopolyhedrovirus, acmnpv, by a combination of pcr and transformation-associated recombination in yeast. the synthetic genome, designated acmnpv-wtv-synl, is 145 299 bp comprising the complete genome of acmnpv except for the hr4a locus that was replaced with an similar to 11.5 kb cassette of bacterial and yeast artificial chromosomal elements and an egfp gene. sf9 insect cells were transfected with acmnpv-wiv-synl dna and progeny virus was examined by electron microscopy, and assayed in one-step growth curves and oral infectivity. the results conclusively showed that the rescued virus acmnpv-wiv-synl had structural and biological properties comparable to the parental virus. we validated a proof of concept that a bona fide baculovirus can be synthesized. the new platform allows manipulation at any or multiple loci and will facilitate future studies such as identifying the minimal baculovirus genome and construction of better expression vectors. this is the largest dna virus synthesized so far, and its success is likely to be the impetus to stimulate the fields of other large dna viruses such as herpesviruses and poxviruses. |
| WOS关键词 | RESPIRATORY SYNDROME VIRUS ; ESCHERICHIA-COLI ; GENOME ; GENERATION ; POLIOVIRUS ; RECONSTRUCTION ; INSECTICIDES ; SEQUENCES ; VACCINE ; CDNA |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000406356100031 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373402 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Deng, Fei; Hu, Zhihong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Great Lakes Forestry Ctr, Lab Mol Virol, Sault Ste Marie, ON P6A 2E5, Canada 4.Wageningen Univ, Lab Virol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Shang, Yu,Wang, Manli,Xiao, Gengfu,et al. Construction and rescue of a functional synthetic baculovirus[J]. Acs synthetic biology,2017,6(7):1393-1402. |
| APA | Shang, Yu.,Wang, Manli.,Xiao, Gengfu.,Wang, Xi.,Hou, Dianhai.,...&Hu, Zhihong.(2017).Construction and rescue of a functional synthetic baculovirus.Acs synthetic biology,6(7),1393-1402. |
| MLA | Shang, Yu,et al."Construction and rescue of a functional synthetic baculovirus".Acs synthetic biology 6.7(2017):1393-1402. |
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来源：武汉病毒研究所
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