Live cell imaging of single genomic loci with quantum dot-labeled tales
文献类型：期刊论文
| 作者 | Ma, Yingxin1,3; Wang, Mingxiu1; Li, Wei1; Zhang, Zhiping1; Zhang, Xiaowei1; Tan, Tianwei3; Zhang, Xian-En2; Cui, Zongqiang1 |
| 刊名 | Nature communications
 |
| 出版日期 | 2017-05-08 |
| 卷号 | 8页码:8 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms15318 |
| 通讯作者 | Zhang, xian-en(x.zhang@wh.iov.cn) ; Cui, zongqiang(czq@wh.iov.cn) |
| 英文摘要 | Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. visualization of single genomic loci in live human cells is currently of great interest, yet it remains challenging. here, we describe a strategy for live cell imaging of single genomic loci by combining transcription activator-like effectors (tales) with a quantum dot labelling technique. we design and select a pair of tales that specifically target hiv-1 proviral dna sequences, and use bioorthogonal ligation reactions to label them with different colour quantum dots (qds). these qd-labelled tales are able to enter the cell nucleus to provide fluorescent signals to identify single gene loci. based on the co-localization of the pair of different coloured qd-labelled tales, we determine and map single-copy hiv-1 provirus loci in human chromosomes in live host cells. |
| WOS关键词 | DNA-SEQUENCES ; LIVING CELLS ; VISUALIZATION ; ACTIVATOR ; EFFECTORS ; DYNAMICS ; FISH |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000400863400001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373403 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Xian-En; Cui, Zongqiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, CAS Ctr Biol Macromol, Natl Key Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 3.Beijing Univ Chem Technol, Beijing Key Lab Bioproc, Coll Life Sci & Technol, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Yingxin,Wang, Mingxiu,Li, Wei,et al. Live cell imaging of single genomic loci with quantum dot-labeled tales[J]. Nature communications,2017,8:8. |
| APA | Ma, Yingxin.,Wang, Mingxiu.,Li, Wei.,Zhang, Zhiping.,Zhang, Xiaowei.,...&Cui, Zongqiang.(2017).Live cell imaging of single genomic loci with quantum dot-labeled tales.Nature communications,8,8. |
| MLA | Ma, Yingxin,et al."Live cell imaging of single genomic loci with quantum dot-labeled tales".Nature communications 8(2017):8. |
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来源：武汉病毒研究所
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