The formation and modification of chromatin-like structure of human parvovirus b19 regulate viral genome replication and rna processing
文献类型：期刊论文
| 作者 | Xu, Huanzhou1,2; Hao, Sujuan1,2; Zhang, Junmei1,2; Chen, Zhen1; Wang, Hanzhong1; Guan, Wuxiang1 |
| 刊名 | Virus research
 |
| 出版日期 | 2017-03-15 |
| 卷号 | 232页码:134-138 |
| ISSN号 | 0168-1702 |
| 关键词 | B19v Chromatin-like structure Dac Replication Rna splicing |
| DOI | 10.1016/j.virusres.2017.03.001 |
| 通讯作者 | Guan, wuxiang(guanwx@wh.iov.cn) |
| 英文摘要 | B19 virus (b19v) is a single stranded virus in the genus of erythroparvovirus in the family of parvoviridae. one of the limiting steps of b19v infection is the replication of viral genome which affected the alternative processing of its rna. minute virus of mice (mvm) and adeno-associated virus (aav) has been reported to form chromatin-like structure within hours after infection of cells. however, the role of chromatin-like structure is unclear. in the present study, we found that b19v formed chromatin-like structure after 12 h when b19v infectious clone was co-transfected with phelper plasmid to hek293t cells. interestingly, the inhibitor of dna methyl-transferase (5-aza-2'-deoxycytidine, dac) inhibited not only the formation of chromatin-like structure, but also the replication of the viral genomic dna. more importantly, the splicing of the second intron at splice acceptor sites (a2-1, and a2-2) were reduced and polyadenylation at (pa)p increased when transfected hek293t cells were treated with dac. our results showed that the formation and modification of chromatin-like structure are a new layer to regulate b19v gene expression and rna processing. (c) 2017 elsevier b.v. all rights reserved. |
| WOS关键词 | DNA-REPLICATION ; INTERNAL POLYADENYLATION ; IMMUNE-RESPONSE ; INFECTED-CELLS ; VIRUS ; PROTEIN ; PROMOTER ; NS1 ; 5-AZA-2'-DEOXYCYTIDINE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000400532700017 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373404 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Guan, Wuxiang |
| 作者单位 | 1.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Huanzhou,Hao, Sujuan,Zhang, Junmei,et al. The formation and modification of chromatin-like structure of human parvovirus b19 regulate viral genome replication and rna processing[J]. Virus research,2017,232:134-138. |
| APA | Xu, Huanzhou,Hao, Sujuan,Zhang, Junmei,Chen, Zhen,Wang, Hanzhong,&Guan, Wuxiang.(2017).The formation and modification of chromatin-like structure of human parvovirus b19 regulate viral genome replication and rna processing.Virus research,232,134-138. |
| MLA | Xu, Huanzhou,et al."The formation and modification of chromatin-like structure of human parvovirus b19 regulate viral genome replication and rna processing".Virus research 232(2017):134-138. |
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来源：武汉病毒研究所
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