Molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses
文献类型：期刊论文
| 作者 | Waruhiu, Cecilia1,2; Ommeh, Sheila3; Obanda, Vincent4; Agwanda, Bernard5; Gakuya, Francis4; Ge, Xing-Yi1,2; Yang, Xing-Lou1,2; Wu, Li-Jun1; Zohaib, Ali1; Hu, Ben1,2 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 32期号:2页码:101-114 |
| ISSN号 | 1674-0769 |
| 关键词 | Astroviruses (astvs) Calicivirus (calvs) Rotavirus a 229-e-like bat coronavirus |
| DOI | 10.1007/s12250-016-3930-2 |
| 通讯作者 | Ommeh, sheila(sommeh@jkuat.ac.ke) ; Shi, zheng-li(zlshi@wh.iov.cn) |
| 英文摘要 | This is the first country-wide surveillance of bat-borne viruses in kenya spanning from 2012-2015 covering sites perceived to have medium to high level bat-human interaction. the objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of kenyan bats. we screened for both dna and rna viruses; specifically, astroviruses (astvs), adenoviruses (advs), caliciviruses (calvs), coronaviruses (covs), flaviviruses, filoviruses, paramyxoviruses (pmvs), polyomaviruses (pyvs) and rotaviruses. we used family-specific primers, amplicon sequencing and further characterization by phylogenetic analysis. except for filoviruses, eight virus families were detected with varying distributions and positive rates across the five regions (former provinces) studied. astvs (12.83%), covs (3.97%), pmv (2.4%), adv (2.26%), pyv (1.65%), calvs (0.29%), rotavirus (0.19%) and flavivirus (0.19%). novel calvs were detected in rousettus aegyptiacus and mops condylurus while novel rotavirus-a-related viruses were detected in taphozous bats and r. aegyptiacus. the two rotavirus a (rva) strains detected were highly related to human strains with vp6 genotypes i2 and i16. genotype i16 has previously been assigned to human rva-strain b10 from kenya only, which raises public health concern, particularly considering increased human-bat interaction. additionally, 229e-like bat covs were detected in samples originating from hipposideros bats roosting in sites with high human activity. our findings confirm the presence of diverse viruses in kenyan bats while providing extended knowledge on bat virus distribution. the detection of viruses highly related to human strains and hence of public health concern, underscores the importance of continuous surveillance. |
| WOS关键词 | EMERGING INFECTIOUS-DISEASES ; HUMAN CORONAVIRUS 229E ; GROUP-A ROTAVIRUS ; EBOLA-VIRUS ; FRUIT BATS ; CHINA ; SARS ; RNA ; CLASSIFICATION ; IDENTIFICATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000400393900001 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373405 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Ommeh, Sheila; Shi, Zheng-Li |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430071, Peoples R China 3.Jomo Kenyatta Univ Sci & Technol, Inst Biotechnol Res, Nairobi 00200, Kenya 4.Kenya Wildlife Serv, Vet Serv Dept, Nairobi 00100, Kenya 5.Natl Museums Kenya, Mammal Sect, Nairobi 00100, Kenya |
| 推荐引用方式 GB/T 7714 | Waruhiu, Cecilia,Ommeh, Sheila,Obanda, Vincent,et al. Molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses[J]. Virologica sinica,2017,32(2):101-114. |
| APA | Waruhiu, Cecilia.,Ommeh, Sheila.,Obanda, Vincent.,Agwanda, Bernard.,Gakuya, Francis.,...&Shi, Zheng-Li.(2017).Molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses.Virologica sinica,32(2),101-114. |
| MLA | Waruhiu, Cecilia,et al."Molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses".Virologica sinica 32.2(2017):101-114. |
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来源：武汉病毒研究所
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