Single-particle tracking of human immunodeficiency virus type 1 productive entry into human primary macrophages
文献类型：期刊论文
| 作者 | Li, Qin1,2; Li, Wei2; Yin, Wen2; Guo, Jia4; Zhang, Zhi-Ping2; Zeng, Dejun2; Zhang, Xiaowei2; Wu, Yuntao4; Zhang, Xian-En3; Cui, Zongqiang2 |
| 刊名 | Acs nano
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 11期号:4页码:3890-3903 |
| 关键词 | Quantum dots Single-particle tracking Human primary macrophages Productive entry Endosomal fusion Actin |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.7b00275 |
| 通讯作者 | Wu, yuntao(ywu8@gmu.edu) ; Zhang, xian-en(zhangxe@ibp.ac.cn) ; Cui, zongqiang(czq@wh.iov.cn) |
| 英文摘要 | Macrophages are one of the major targets of human immunodeficiency virus (hiv-1), but the viral entry pathway remains poorly understood in these cells. noninvasive virus labeling and single-virus tracking are effective tools for studying virus entry. here, we constructed a quantum dot (qd)-encapsulated infectious hiv-1 particle to track viral entry at a single-particle level in live human primary macrophages. qds were encapsulated in hiv-1 virions by incorporating viral accessory protein vpr-conjugated qds during virus assembly. with the hiv-1 particles encapsulating qds, we monitored the early phase of viral infection in real time and observed that, during infection, hiv-1 was endocytosed in a clathrin-mediated manner; the particles were translocated into rab5a-positive endosomes, and the core was released into the cytoplasm by viral envelope-mediated endosomal fusion. drug inhibition assays verified that endosome fusion contributes to hiv-1 productive infection in primary macrophages. additionally, we observed that a dynamic actin cytoskeleton is critical for hiv-1 entry and intracellular migration in primary macrophages. hiv-1 dynamics and infection could be blocked by multiple different actin inhibitors. our study revealed a productive entry pathway in macrophages that requires both endosomal function and actin dynamics, which may assist in the development of inhibitors to block the hiv entry in macrophages. |
| WOS关键词 | CLATHRIN-MEDIATED ENDOCYTOSIS ; CD4(+) T-CELLS ; QUANTUM DOTS ; ENDOSOME FUSION ; HIV-1 INFECTION ; ENVELOPED VIRUS ; LIVING CELLS ; HOST-CELLS ; INHIBITION ; MONOCYTES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000400233200048 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373406 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wu, Yuntao; Zhang, Xian-En; Cui, Zongqiang |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 4.George Mason Univ, Dept Mol & Microbiol, Natl Ctr Biodef & Infect Dis, Manassas, VA 20110 USA |
| 推荐引用方式 GB/T 7714 | Li, Qin,Li, Wei,Yin, Wen,et al. Single-particle tracking of human immunodeficiency virus type 1 productive entry into human primary macrophages[J]. Acs nano,2017,11(4):3890-3903. |
| APA | Li, Qin.,Li, Wei.,Yin, Wen.,Guo, Jia.,Zhang, Zhi-Ping.,...&Cui, Zongqiang.(2017).Single-particle tracking of human immunodeficiency virus type 1 productive entry into human primary macrophages.Acs nano,11(4),3890-3903. |
| MLA | Li, Qin,et al."Single-particle tracking of human immunodeficiency virus type 1 productive entry into human primary macrophages".Acs nano 11.4(2017):3890-3903. |
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来源：武汉病毒研究所
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