Improving baculovirus infectivity by efficiently embedding enhancing factors into occlusion bodies
文献类型：期刊论文
| 作者 | Yang, Shili1,2; Zhao, Lijuan1,2; Ma, Ruipeng1; Fang, Wei1; Hu, Jia1; Lei, Chengfeng1; Sun, Xiulian1 |
| 刊名 | Applied and environmental microbiology
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 83期号:14页码:12 |
| 关键词 | Acmnpv Dimidiate polyhedrin Embedding Enhancin Gp37 Infectivity |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.00595-17 |
| 通讯作者 | Sun, xiulian(sunxl@wh.iov.cn) |
| 英文摘要 | The relatively low infectivity of baculoviruses to their host larvae limits their use as insecticidal agents on a larger scale. in the present study, a novel strategy was developed to efficiently embed foreign proteins into autographa californica multiple nucleopolyhedrovirus (acmnpv) occlusion bodies (obs) to achieve stable expression of foreign proteins and to improve viral infectivity. a recombinant acmnpv bacmid was constructed by expressing the 150-amino-acid (aa) n-terminal segment of polyhedrin under the control of the p10 promoter and the remaining c-terminal 95-aa segment under the control of the polyhedrin promoter. the recombinant virus formed obs in spodoptera frugiperda 9 cells, in which the occlusion-derived viruses were embedded in a manner similar to that for wild-type acmnpv. next, the 95-aa polyhedrin c terminus was fused to enhanced green fluorescent protein, and the recombinant acmnpv formed fluorescent green obs and was stably passaged in vitro and in vivo. the acmnpv recombinants were further modified by fusing truncated agrotis segetum granulovirus enhancin or truncated cydia pomonella granulovirus orf13 (gp37) to the c-terminal 95 aa of polyhedrin, and both recombinants were able to form normal obs. bioactivity assays indicated that the median lethal concentrations of these two acmnpv recombinants were 3-to 5-fold lower than that of the control virus. these results suggest that embedding enhancing factors in baculovirus obs by use of this novel technique may promote efficient and stable foreign protein expression and significantly improve baculovirus infectivity. importance baculoviruses have been used as bioinsecticides for over 40 years, but their relatively low infectivity to their host larvae limits their use on a larger scale. it has been reported that it is possible to improve baculovirus infectivity by packaging enhancing factors within baculovirus occlusion bodies (obs); however, so far, the packaging efficiency has been low. in this article, we describe a novel strategy for efficiently embedding foreign proteins into acmnpv obs by expressing nand c-terminal (dimidiate) polyhedrin fragments (150 and 95 amino acids, respectively) as fusions to foreign proteins under the control of the p10 and polyhedrin promoters, respectively. when this strategy was used to embed an enhancing factor (enhancin or gp37) into the baculovirus obs, 3-to 5-fold increases in baculoviral infectivity were observed. this novel strategy has the potential to create an efficient protein expression system and a highly efficient virus-based system for insecticide production in the future. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; RECOMBINANT BACULOVIRUSES ; PERITROPHIC MEMBRANE ; TRICHOPLUSIA NI ; PROTEIN ; REVEALS ; BINDING ; MIDGUT ; MUTANT ; LARVAE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000404604600007 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373418 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Sun, Xiulian |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Shili,Zhao, Lijuan,Ma, Ruipeng,et al. Improving baculovirus infectivity by efficiently embedding enhancing factors into occlusion bodies[J]. Applied and environmental microbiology,2017,83(14):12. |
| APA | Yang, Shili.,Zhao, Lijuan.,Ma, Ruipeng.,Fang, Wei.,Hu, Jia.,...&Sun, Xiulian.(2017).Improving baculovirus infectivity by efficiently embedding enhancing factors into occlusion bodies.Applied and environmental microbiology,83(14),12. |
| MLA | Yang, Shili,et al."Improving baculovirus infectivity by efficiently embedding enhancing factors into occlusion bodies".Applied and environmental microbiology 83.14(2017):12. |
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来源：武汉病毒研究所
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