Rapid and selective detection of e. coli o157:h7 combining phagomagnetic separation with enzymatic colorimetry
文献类型：期刊论文
| 作者 | Zhang, Yun1,2,3; Yan, Chenghui1,2; Yang, Hang1; Yu, Junping1; Wei, Hongping1,4 |
| 刊名 | Food chemistry
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 234页码:332-338 |
| 关键词 | E. coli o157:h7 Magnetic beads Bacteriophage Detection Specificity |
| ISSN号 | 0308-8146 |
| DOI | 10.1016/j.foodchem.2017.05.013 |
| 通讯作者 | Yu, junping(yujp@wh.iov.cn) ; Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | Mammal igg antibodies are normally used in conventional immunoassays for e. coli o157:h7, which could lead to false positive results from the presence of protein a producing s. aureus. in this study, a natural specific bacteriophage was isolated and then conjugated with magnetic beads as a capture element in a sandwich format for the rapid and selective detection of e. coli o157:h7. to the best of our knowledge, it was the first time to utilize a natural bacteriophage to develop a phagomagnetic separation combined with colorimetric assay for e. coli o157:h7. the method has an overall time less than 2 h with a detection limit of 4.9 x 10(4) cfu/ml. no interference from s. aureus was observed. furthermore, the proposed method was successfully applied to detect e. coli o157:h7 in spiked skim milk. the proposed detection system provided a potential method for e. coli o157:h7 and other pathogenic bacteria in food samples. (c) 2017 elsevier ltd. all rights reserved. |
| WOS关键词 | ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY ; LINKED-IMMUNOSORBENT-ASSAY ; SURFACE-PLASMON RESONANCE ; ESCHERICHIA-COLI ; SENSITIVE DETECTION ; GROUND-BEEF ; IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENCE ; HEMORRHAGIC COLITIS ; BACTERIOPHAGE ; O157-H7 |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Nutrition & Dietetics |
| WOS类目 | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000403510700043 |
| 出版者 | ELSEVIER SCI LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373422 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yu, Junping; Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Xinxiang Med Univ, Sch Lab Med, Henan Collaborat Innovat Ctr Mol Diag & Lab Med, Xinxiang 453003, Henan, Peoples R China 4.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yun,Yan, Chenghui,Yang, Hang,et al. Rapid and selective detection of e. coli o157:h7 combining phagomagnetic separation with enzymatic colorimetry[J]. Food chemistry,2017,234:332-338. |
| APA | Zhang, Yun,Yan, Chenghui,Yang, Hang,Yu, Junping,&Wei, Hongping.(2017).Rapid and selective detection of e. coli o157:h7 combining phagomagnetic separation with enzymatic colorimetry.Food chemistry,234,332-338. |
| MLA | Zhang, Yun,et al."Rapid and selective detection of e. coli o157:h7 combining phagomagnetic separation with enzymatic colorimetry".Food chemistry 234(2017):332-338. |
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来源：武汉病毒研究所
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