Requirement of cytosolic phospholipase a2 gamma in lipid droplet formation
文献类型：期刊论文
| 作者 | Su, Xi1,2; Liu, Shuhui1,2; Zhang, Xianwen1,2; Lam, Sin Man4; Hu, Xue1; Zhou, Yuan1; Chen, Jizheng1; Wang, Yun1; Wu, Chunchen1; Shui, Guanghou4 |
| 刊名 | Biochimica et biophysica acta-molecular and cell biology of lipids
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 1862期号:7页码:692-705 |
| 关键词 | Lipid droplet Hcv Cytosolic phospholipase a2 gamma Pla2g4c Assemble Steatosis |
| ISSN号 | 1388-1981 |
| DOI | 10.1016/j.bbalip.2017.03.007 |
| 通讯作者 | Pei, rongjuan(rongjuan_pei@wh.iov.cn) ; Chen, xinwen(chenxw@wh.iov.cn) |
| 英文摘要 | Lipid droplet (ld) accumulation in hepatocytes is a typical character of steatosis. hepatitis c virus (hcv) infection, one of the risk factors related to steatosis, induced ld accumulation in cultured cells. however, the mechanisms of which hcv induce ld formation are not fully revealed. previously we identified cytosolic phospholipase a2 gamma (pla2g4c) as a host factor upregulated by hcv infection and involved in hcv replication. here we further revealed that pla2g4c plays an important role in ld biogenesis and refined the functional analysis of pla2g4c in ld biogenesis and hcv assembly. ld formation upon fatty acid and hcv stimulation in pla2g4c knockdown cells was impaired and could not be restored by complementation with pla2g4a. pla2g4c was tightly associated in the membrane with the domain around the amino acid residues 260-292, normally in er but relocated into lds upon oleate stimulation. mutant pla2g4c without enzymatic activity was not able to restore ld formation in pla2g4c knockdown cells. thus, both the membrane attachment and the enzymatic activity of pla2g4c were required for its function in ld formation. the participation of pla2g4c in ld formation is correlated with its involvement in hcv assembly. finally, pla2g4c overexpression itself led to ld formation in hepatic cells and enhanced ld accumulation in the liver of high-fat diet (hfd)-fed mice, suggesting its potential role in fatty liver disease. |
| WOS关键词 | HEPATITIS-C VIRUS ; DIFFERENTIATION-RELATED PROTEIN ; CIDE PROTEINS ; A(2) ; MEMBRANE ; BIOGENESIS ; ACTIVATION ; STEATOSIS ; STORAGE ; DISEASE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000402359600006 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373425 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Pei, Rongjuan; Chen, Xinwen |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Duisburg Essen, Univ Hosp Essen, Dept Infect Dis, Essen, Germany 4.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Xi,Liu, Shuhui,Zhang, Xianwen,et al. Requirement of cytosolic phospholipase a2 gamma in lipid droplet formation[J]. Biochimica et biophysica acta-molecular and cell biology of lipids,2017,1862(7):692-705. |
| APA | Su, Xi.,Liu, Shuhui.,Zhang, Xianwen.,Lam, Sin Man.,Hu, Xue.,...&Chen, Xinwen.(2017).Requirement of cytosolic phospholipase a2 gamma in lipid droplet formation.Biochimica et biophysica acta-molecular and cell biology of lipids,1862(7),692-705. |
| MLA | Su, Xi,et al."Requirement of cytosolic phospholipase a2 gamma in lipid droplet formation".Biochimica et biophysica acta-molecular and cell biology of lipids 1862.7(2017):692-705. |
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来源：武汉病毒研究所
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