Deltabaculoviruses encode a functional type i budded virus envelope fusion protein
文献类型：期刊论文
| 作者 | Wang, Manli1,2; Shen, Shu2; Wang, Hualin2; Hu, Zhihong2; Becnel, James3; Vlak, Just M.1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 98期号:4页码:847-852 |
| 关键词 | Deltabaculovirus Cuninpv F protein Function Fusogenicity |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/jgv.0.000745 |
| 通讯作者 | Vlak, just m.(just.vlak@wur.nl) |
| 英文摘要 | Envelope fusion proteins (f proteins) are major constituents of budded viruses (bvs) of alpha-and betabaculoviruses (baculoviridae) and are essential for the systemic infection of insect larvae and insect cell culture. an f homologue gene is absent in gammabaculoviruses. here we characterized the putative f-homologue (cuni-f), encoded by (orf) 104 of culex nigripalpus nucleopolyhedrovirus (cuninpv), the only deltabaculovirus member. when expressed alone, this protein seems to locate on the cell surface and is able to induce cell-cell fusion. when expressed by an alphabaculovirus (autographa california nucleopolyhedrovirus), it was found to be incorporated into bvs. western blot analyses detected the uncleaved cuni-f-0 and the furin-cleaved f-1 forms. treatment of infected cells with tunicamycin showed that cuni-f contains n-glycans. mutagenesis analysis identified the canonical furin cleavage site (126)rarr(129) as being responsible for the cleavage of cuni-f in insect cells. the collective evidence suggests that cuninpv encodes a functional f protein. |
| WOS关键词 | AUTOGRAPHA-CALIFORNICA NUCLEOPOLYHEDROVIRUS ; ARMIGERA SINGLE NUCLEOPOLYHEDROVIRUS ; N-LINKED GLYCOSYLATION ; MULTICAPSID NUCLEOPOLYHEDROVIRUS ; CULEX-NIGRIPALPUS ; F-PROTEIN ; BACULOVIRUS ; GP64 ; INFECTIVITY ; HOMOLOG |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000401905000034 |
| 出版者 | MICROBIOLOGY SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373426 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, Just M. |
| 作者单位 | 1.Wageningen Univ, Lab Virol, Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 3.ARS, USDA, CMAVE, Gainesville, FL USA |
| 推荐引用方式 GB/T 7714 | Wang, Manli,Shen, Shu,Wang, Hualin,et al. Deltabaculoviruses encode a functional type i budded virus envelope fusion protein[J]. Journal of general virology,2017,98(4):847-852. |
| APA | Wang, Manli,Shen, Shu,Wang, Hualin,Hu, Zhihong,Becnel, James,&Vlak, Just M..(2017).Deltabaculoviruses encode a functional type i budded virus envelope fusion protein.Journal of general virology,98(4),847-852. |
| MLA | Wang, Manli,et al."Deltabaculoviruses encode a functional type i budded virus envelope fusion protein".Journal of general virology 98.4(2017):847-852. |
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来源：武汉病毒研究所
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