Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of staphylococcus aureus
文献类型：期刊论文
| 作者 | Yan, Chenghui1,2; Zhang, Yun1,2,3; Yang, Hang1; Yu, Junping1; Wei, Hongping1,4 |
| 刊名 | Talanta
 |
| 出版日期 | 2017-08-01 |
| 卷号 | 170页码:291-297 |
| 关键词 | Staphylococcus aureus Magnetic separation Bacteriophage Detection Specificity |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2017.04.007 |
| 通讯作者 | Yu, junping(yujp@wh.iov.cn) ; Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | A staphylococcus aureus (s. aureus)-specific lytic bacteriophage p-s. aureus-9, isolated from an environmental water sample, was assembled on magnetic beads for capturing s. aureus from samples through magnetic separation. horseradish peroxidase (hrp) labeled immunoglobulin (igg) antibodies were used to detect the captured s. aureus by reacting with protein a on s. aureus followed by colorimetric signals, which were generated from the catalytic reaction between hrp and the substrate 3,3',5,5'-tetramethylbenzidine (tmb). under optimal conditions, the calibration curve was linear from 1.0x10(4) to 1.0x10(6) cfu ml(-1). the limit of detection (lod) for the assay was 2.47x10(3) cfu mirl and 8.86x10(3) cfu ml(-1) of s. aureus in pbs and apple juice, respectively. moreover, the whole assay revealed outstanding specificity towards s. aureus, without any interference of common pathogenic bacteria, and can be completed within 90 min without any pre-enrichment. as far as known, it was the first time to detect s. aureus based on the double site recognition of bacteriophage and mammal igg. the novel approach has shown good potentials for a rapid, specific, cheap and simple detection of s. aureus in food samples. |
| WOS关键词 | PATHOGEN DETECTION ; RAPID DETECTION ; BACTERIA ; BIOSENSORS ; NANOPARTICLES ; FOOD ; AMPLIFICATION ; SEDIMENTATION ; RECOGNITION ; LATEX |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000402343200040 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373429 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yu, Junping; Wei, Hongping |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Special Pathogens & Biosafety, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Xinxiang Med Univ, Henan Collaborat Innovat Ctr Mol Diag & Lab Med, Sch Lab Med, Xinxiang 453003, Henan, Peoples R China 4.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Chenghui,Zhang, Yun,Yang, Hang,et al. Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of staphylococcus aureus[J]. Talanta,2017,170:291-297. |
| APA | Yan, Chenghui,Zhang, Yun,Yang, Hang,Yu, Junping,&Wei, Hongping.(2017).Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of staphylococcus aureus.Talanta,170,291-297. |
| MLA | Yan, Chenghui,et al."Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of staphylococcus aureus".Talanta 170(2017):291-297. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。