Genome-wide identification and characterization of genes encoding cyclohexylamine degradation in a novel cyclohexylamine-degrading bacterial strain of pseudomonas plecoglossicida nyz12
文献类型：期刊论文
| 作者 | Yan, Da-Zhong1; Li, Xin1; Li, Cun-Zhi1; Mao, Ling-Qi1; Chi, Xiang-Qun2; Zhou, Ning-Yi2,3,4; Liu, Dong-Yan5,6 |
| 刊名 | Journal of biotechnology
 |
| 出版日期 | 2017-06-10 |
| 卷号 | 251页码:166-173 |
| ISSN号 | 0168-1656 |
| 关键词 | Pseudomonas plecoglossicida nyz12 Catabolism Cyclohexylamine Cyclohexylamine oxidase Cyclohexanone |
| DOI | 10.1016/j.jbiotec.2017.04.036 |
| 通讯作者 | Yan, da-zhong(yandz6808@163.com) |
| 英文摘要 | The gram-negative strain of pseudomonas plecoglossicida nyz12 isolated from soil has the ability to degrade cyclohexylamine (cham). the genes encoding cham degradation by gram-negative bacteria, however, have not been reported previously. in this study, orfs predicted to encode cham degradation by nyz12 were identified by bioinformatics analysis. differential expression of the proposed orfs was analyzed via rna-seq and quantitative reverse transcription-pcr (qrt-pcr), using rna extracted from nyz12 cultured with or without cham addition. one cham-inducible orf, rk21_02867 predicted to encode a cyclohexanone monooxygenase (chnb) was disrupted, as were five orfs, rk21_00425, rk21_02631, rk21_04207, rk21_04637 and rk21_05539, that had weak homology to the only known cyclohexylamine oxidase (chao encoded by chaa) found in brevibacterium oxydans ih-35a. we also found that a tandem array of five orfs (rk21_02866-02870) shared homology with those in an operon responsible for oxidation of cyclohexanone to adipic acid, although the orfs in strain nyz12 were arranged in a different order with previously found in cyclohexane, cyclohexanol or cyclohexanone degradation strains. the orfs in this cluster were all up-regulated when cham was supplied as the sole carbon source. when one of these five genes, rk21_02867 encoding cyclohexanone (chnone) monooxygenase, was knocked out, nyz12 could not grow on cham, but it accumulated equimolar amounts of chnone. our results show that strain nyz12 metabolized cham directly to chnone which was then further metabolized to adipate. despite clearly identifying genes encoding the steps for metabolism of cham metabolites, not every one of the putative chaas was differentially expressed in the presence of cham and deletion of each one individually did not completely eliminate the capacity of nyz12 to degrade cham, though it did reduce its growth in several instances. our results suggest that there is genetic redundancy encoding the initial step in the oxidation of cham to chnone in nyz12 and that its chaos differ considerably from the chaa, originally described in brevibacterium oxydans ih-35a. |
| WOS关键词 | BREVIBACTERIUM-OXYDANS IH-35A ; MONOAMINE-OXIDASE ; CYCLOHEXANOL ; PURIFICATION ; METABOLISM ; INHIBITORS ; DISCOVERY ; ISLANDS ; CLONING |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000402470300023 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373433 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yan, Da-Zhong |
| 作者单位 | 1.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China 5.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China 6.Shanghai Normal Univ, Urban Ecol & Environm Res Ctr, Shanghai 200234, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Da-Zhong,Li, Xin,Li, Cun-Zhi,et al. Genome-wide identification and characterization of genes encoding cyclohexylamine degradation in a novel cyclohexylamine-degrading bacterial strain of pseudomonas plecoglossicida nyz12[J]. Journal of biotechnology,2017,251:166-173. |
| APA | Yan, Da-Zhong.,Li, Xin.,Li, Cun-Zhi.,Mao, Ling-Qi.,Chi, Xiang-Qun.,...&Liu, Dong-Yan.(2017).Genome-wide identification and characterization of genes encoding cyclohexylamine degradation in a novel cyclohexylamine-degrading bacterial strain of pseudomonas plecoglossicida nyz12.Journal of biotechnology,251,166-173. |
| MLA | Yan, Da-Zhong,et al."Genome-wide identification and characterization of genes encoding cyclohexylamine degradation in a novel cyclohexylamine-degrading bacterial strain of pseudomonas plecoglossicida nyz12".Journal of biotechnology 251(2017):166-173. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。