Detection of alpha- and betacoronaviruses in rodents from yunnan, china
文献类型：期刊论文
| 作者 | Ge, Xing-Yi1,4; Yang, Wei-Hong2; Zhou, Ji-Hua2; Li, Bei1; Zhang, Wei1; Shi, Zheng-Li1; Zhang, Yun-Zhi2,3 |
| 刊名 | Virology journal
 |
| 出版日期 | 2017-05-26 |
| 卷号 | 14页码:11 |
| 关键词 | Rodent Coronavirus Genetic diversity |
| ISSN号 | 1743-422X |
| DOI | 10.1186/s12985-017-0766-9 |
| 通讯作者 | Shi, zheng-li(zlshi@wh.iov.cn) ; Zhang, yun-zhi(zhangyunzhi1818@163.com) |
| 英文摘要 | Background: rodents represent the most diverse mammals on the planet and are important reservoirs of human pathogens. coronaviruses infect various animals, but to date, relatively few coronaviruses have been identified in rodents worldwide. the evolution and ecology of coronaviruses in rodent have not been fully investigated. results: in this study, we collected 177 intestinal samples from thress species of rodents in jianchuan county, yunnan province, china. alphacoronavirus and betacoronavirus were detected in 23 rodent samples from three species, namely apodemus chevrieri (21/98), eothenomys fidelis (1/62), and apodemus ilex (1/17). we further characterized the full-length genome of an alphacoronavirus from the a. chevrieri rat and named it as accov-jc34. the accov-jc34 genome was 27,649 nucleotides long and showed a structure similar to the hku2 bat coronavirus. comparing the normal transcription regulatory sequence (trs), 3 variant trs sequences upstream the spike (s), orf3, and orf8 genes were found in the genome of accov-jc34. in the conserved replicase domains, accov-jc34 was most closely related to rattus norvegicus coronavirus lnrv but diverged from other alphacoronaviruses, indicating that accov-jc34 and lnrv may represent a novel alphacoronavirus species. however, the s and nucleocapsid proteins showed low similarity to those of lrnv, with 66.5 and 77.4% identities, respectively. phylogenetic analysis revealed that the s genes of accov-jc34, lrnv, and hku2 formed a distinct lineage with all known coronaviruses. conclusions: both alphacoronaviruses and betacoronaviruses were detected in apodemus chevrieri in the yunnan province of china, indicating that apodemus chevrieri is an important host for coronavirus. several new features were identified in the genome of an apodemus chevrieri coronavirus. the phylogenetic distance to other coronaviruses suggests a variable origin and evolutionary route of the s genes of accov-jc34, lrnv, and hku2. these results indicate that the diversity of rodent coronaviruses is much higher than previously expected. further surveillance and functional studies of these coronaviruses will help to better understand the importance of rodent as host for coronaviruses. |
| WOS关键词 | RECEPTOR-BINDING DOMAIN ; ACUTE RESPIRATORY SYNDROME ; EPIDEMIC DIARRHEA VIRUS ; CHEVRIERS FIELD-MOUSE ; CYTOCHROME-B GENE ; SPIKE PROTEIN ; SYNDROME CORONAVIRUS ; APODEMUS-CHEVRIERI ; PHYLOGENETIC TREE ; BAT CORONAVIRUSES |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000402156100001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373438 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Shi, Zheng-Li; Zhang, Yun-Zhi |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China 2.Yunnan Inst Endem Dis Control & Prevent, Yunnan Prov Key Lab Zoonosis Control & Prevent, Dali 671000, Peoples R China 3.Dali Univ, Sch Publ Hlth, Dali 671000, Peoples R China 4.Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Xing-Yi,Yang, Wei-Hong,Zhou, Ji-Hua,et al. Detection of alpha- and betacoronaviruses in rodents from yunnan, china[J]. Virology journal,2017,14:11. |
| APA | Ge, Xing-Yi.,Yang, Wei-Hong.,Zhou, Ji-Hua.,Li, Bei.,Zhang, Wei.,...&Zhang, Yun-Zhi.(2017).Detection of alpha- and betacoronaviruses in rodents from yunnan, china.Virology journal,14,11. |
| MLA | Ge, Xing-Yi,et al."Detection of alpha- and betacoronaviruses in rodents from yunnan, china".Virology journal 14(2017):11. |
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来源：武汉病毒研究所
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