Japanese encephalitis virus ns5 inhibits type i interferon (ifn) production by blocking the nuclear translocation of ifn regulatory factor 3 and nf-kappa b
文献类型：期刊论文
| 作者 | Ye, Jing1,2,3,4; Chen, Zheng1,2,4; Li, Yunchuan1,2,4; Zhao, Zikai1,2,4; He, Wen1,4; Zohaib, Ali1,2,4; Song, Yunfeng1,2,4; Deng, Chenglin5; Zhang, Bo5; Chen, Huanchun1,2,4 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 91期号:8页码:16 |
| ISSN号 | 0022-538X |
| 关键词 | Japanese encephalitis virus Ns5 Innate immunity Nuclear transport |
| DOI | 10.1128/jvi.00039-17 |
| 通讯作者 | Cao, shengbo(sbcao@mail.hzau.edu.cn) |
| 英文摘要 | The type i interferon (ifn) response is part of the first-line defense against viral infection. to initiate replication, viruses have developed powerful evasion strategies to counteract host ifn responses. in the present study, we found that the japanese encephalitis virus (jev) ns5 protein could inhibit double-stranded rna (dsrna)-induced ifn-beta expression in a dose-dependent manner. our data further demonstrated that jev ns5 suppressed the activation of the ifn transcriptional factors ifn regulatory factor 3 (irf3) and nf-kappa b. however, there was no defect in the phosphorylation of irf3 and degradation of i kappa b, an upstream inhibitor of nf-kappa b, upon ns5 expression, indicating a direct inhibition of the nuclear localization of irf3 and nf-kappa b by ns5. mechanistically, ns5 was shown to interact with the nuclear transport proteins kpna2, kpna3, and kpna4, which competitively blocked the interaction of kpna3 and kpna4 with their cargo molecules, irf3 and p65, a subunit of nf-kappa b, and thus inhibited the nuclear translocation of irf3 and nf-kappa b. furthermore, overexpression of kpna3 and kpna4 restored the activity of irf3 and nf-kappa b and increased the production of ifn-kappa in ns5-expressing or jev-infected cells. additionally, an upregulated replication level of jev was shown upon kpna3 or kpna4 overexpression. these results suggest that jev ns5 inhibits the induction of type i ifn by targeting kpna3 and kpna4. importance jev is the major cause of viral encephalitis in south and southeast asia, with high mortality. however, the molecular mechanisms contributing to the severe pathogenesis are poorly understood. the ability of jev to counteract the host innate immune response is potentially one of the mechanisms responsible for jev virulence. here we demonstrate the ability of jev ns5 to interfere with the dsrna-induced nuclear translocation of irf3 and nf-kappa b by competitively inhibiting the interaction of irf3 and nf-kappa b with nuclear transport proteins. via this mechanism, jev ns5 suppresses the induction of type i ifn and the antiviral response in host cells. these findings reveal a novel strategy for jev to escape the host innate immune response and provide new insights into the pathogenesis of jev. |
| WOS关键词 | WEST-NILE-VIRUS ; INNATE ANTIVIRAL RESPONSE ; RNA POLYMERASE NS5 ; DENGUE VIRUS ; LOCALIZATION SEQUENCES ; SIGNAL-TRANSDUCTION ; RIG-I ; PROTEIN ; ACTIVATION ; ALPHA |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000399472200004 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373442 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Cao, Shengbo |
| 作者单位 | 1.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 2.Huazhong Agr Univ, Coll Vet Med, Lab Anim Virol, Wuhan, Hubei, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China 4.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Hubei, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Jing,Chen, Zheng,Li, Yunchuan,et al. Japanese encephalitis virus ns5 inhibits type i interferon (ifn) production by blocking the nuclear translocation of ifn regulatory factor 3 and nf-kappa b[J]. Journal of virology,2017,91(8):16. |
| APA | Ye, Jing.,Chen, Zheng.,Li, Yunchuan.,Zhao, Zikai.,He, Wen.,...&Cao, Shengbo.(2017).Japanese encephalitis virus ns5 inhibits type i interferon (ifn) production by blocking the nuclear translocation of ifn regulatory factor 3 and nf-kappa b.Journal of virology,91(8),16. |
| MLA | Ye, Jing,et al."Japanese encephalitis virus ns5 inhibits type i interferon (ifn) production by blocking the nuclear translocation of ifn regulatory factor 3 and nf-kappa b".Journal of virology 91.8(2017):16. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。