Global quantitative proteomic analysis profiles host protein expression in response to sendai virus infection
文献类型：期刊论文
| 作者 | Zhu, Sheng-Lin1; Chen, Xi2,3; Wang, Liang-Jie4; Wan, Wei-Wei1; Xin, Qi-Lin1; Wang, Wei1; Xiao, Gengfu1; Zhang, Lei-Ke1 |
| 刊名 | Proteomics
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 17期号:5页码:13 |
| ISSN号 | 1615-9853 |
| 关键词 | Innate immune response Quantitative proteomics Sendai virus Virus-host interaction |
| DOI | 10.1002/pmic.201600239 |
| 通讯作者 | Zhang, lei-ke(zhangleike@wh.iov.cn) |
| 英文摘要 | Sendai virus (sev) is an enveloped nonsegmented negative-strand rna virus that belongs to the genus respirovirus of the paramyxoviridae family. as a model pathogen, sev has been extensively studied to define the basic biochemical and molecular biologic properties of the paramyxoviruses. in addition, sev-infected host cells were widely employed to uncover the mechanism of innate immune response. to identify proteins involved in the sev infection process or the sev-induced innate immune response process, system-wide evaluations of sev-host interactions have been performed. cdna microarray, sirna screening and phosphoproteomic analysis suggested that multiple signaling pathways are involved in sev infection process. here, to study sev-host interaction, a global quantitative proteomic analysis was performed on sev-infected hek 293t cells. a total of 4699 host proteins were quantified, with 742 proteins being differentially regulated. bioinformatics analysis indicated that regulated proteins were mainly involved in "interferon type i (ifn-i) signaling pathway" and "defense response to virus," suggesting that these processes play roles in sev infection. further rnai-based functional studies indicated that the regulated proteins, tripartite motif (trim24) and trim27, affect sev-induced ifn-i production. our data provided a comprehensive view of host cell response to sev and identified host proteins involved in the sev infection process or the sev-induced innate immune response process. |
| WOS关键词 | NF-KAPPA-B ; TRANSCRIPTION FACTOR ACTIVATION ; CELLULAR ANTIVIRAL RESPONSE ; NEWCASTLE-DISEASE VIRUS ; I INTERFERON INDUCTION ; EPITHELIAL-CELLS ; K63-LINKED UBIQUITINATION ; GENE-EXPRESSION ; DENDRITIC CELLS ; RIG-I |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000397390800004 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373455 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, Lei-Ke |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China 2.Wuhan Inst Biotechnol, Lab Biol Mass Spectromet, Wuhan, Peoples R China 3.Wuhan Univ, Med Res Inst, Wuhan, Peoples R China 4.Hubei Univ Educ, Sch Chem & Life Sci, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Sheng-Lin,Chen, Xi,Wang, Liang-Jie,et al. Global quantitative proteomic analysis profiles host protein expression in response to sendai virus infection[J]. Proteomics,2017,17(5):13. |
| APA | Zhu, Sheng-Lin.,Chen, Xi.,Wang, Liang-Jie.,Wan, Wei-Wei.,Xin, Qi-Lin.,...&Zhang, Lei-Ke.(2017).Global quantitative proteomic analysis profiles host protein expression in response to sendai virus infection.Proteomics,17(5),13. |
| MLA | Zhu, Sheng-Lin,et al."Global quantitative proteomic analysis profiles host protein expression in response to sendai virus infection".Proteomics 17.5(2017):13. |
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来源：武汉病毒研究所
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