Identification of an rnase that preferentially cleaves a/g nucleotides
文献类型：期刊论文
| 作者 | Xie, Jumin; Chen, Zhen; Zhang, Xueyan; Chen, Honghe; Guan, Wuxiang |
| 刊名 | Scientific reports
 |
| 出版日期 | 2017-03-21 |
| 卷号 | 7页码:11 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep45207 |
| 英文摘要 | Ribonucleases play an important role in the rna metabolism which is critical for the localization, stability and function of mature rna transcripts. more and more ribonucleases were discovered in recent years with the progress of technology. in the present study, we found that the uncharacterized c19orf43, a novel interacting protein of human telomerase rna (htr), digested t7 transcribed rna, total cellular rna and rna oligos but not dna. thus we named this new rnase as htrir (human telomerase rna interacting rnase). genetic analysis showed that htrir is conserved among eukaryotic species and widely expressed in different cell lines. the rnase activity of htrir works in a broad temperature and ph range while divalent cations are not required. the conserved c-terminus of c19orf43 is necessary for its activity. finally, we found that htrir cleaves all four unpaired rna nucleotides from 5' end or 3' end with higher efficiency for purine bases, which suggested that htrir is an exoribonuclease. taken together, our study showed the first evidence of the novel function of htrir in vitro, which provides clue to study the regulatory mechanism of htr homeostasis in vivo. |
| WOS关键词 | HUMAN TELOMERASE RNA ; BOVINE PANCREATIC RIBONUCLEASE ; SPLICEOSOMAL COMPLEX E ; ACTIVE-SITE ; U2 SNRNP ; 3' END ; PROTEIN ; GENE ; ACETYLATION ; ASSOCIATION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000396853500001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373456 |
| 专题 | 武汉病毒研究所 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, Wuhan 430071, Hunan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Jumin,Chen, Zhen,Zhang, Xueyan,et al. Identification of an rnase that preferentially cleaves a/g nucleotides[J]. Scientific reports,2017,7:11. |
| APA | Xie, Jumin,Chen, Zhen,Zhang, Xueyan,Chen, Honghe,&Guan, Wuxiang.(2017).Identification of an rnase that preferentially cleaves a/g nucleotides.Scientific reports,7,11. |
| MLA | Xie, Jumin,et al."Identification of an rnase that preferentially cleaves a/g nucleotides".Scientific reports 7(2017):11. |
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来源：武汉病毒研究所
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