A simple red blood cell lysis method for the establishment of b lymphoblastoid cell lines
文献类型：期刊论文
| 作者 | Liu, Xi1,2; Xu, Chongfeng2; Duan, Ziyuan2 |
| 刊名 | Jove-journal of visualized experiments
 |
| 出版日期 | 2017 |
| 期号 | 119页码:6 |
| 关键词 | Immunology Issue 119 Ebv Red blood cell lysis method Transformation B-lcls Pbmc Density gradient separation method |
| ISSN号 | 1940-087X |
| DOI | 10.3791/55191 |
| 通讯作者 | Duan, ziyuan(zyduan@genetics.ac.cn) |
| 英文摘要 | A number of methods exist for the transformation of b lymphocytes by the epstein barr virus in vitro into immortalized cell lines. we have developed a new method with a powerful and simple strategy for the establishment of b-lcls, the red blood cell lysis method. this method simplified the pbmc separation procedure with red blood cell removal, and used as little as 0.5 ml of whole blood for establishing ebv-immortalized cell lines, which can proliferate to large cell numbers in a relatively short amount time with a 100% success rate. the method is simple, reliable, time saving, and applicable to treating a large number of the clinical samples. |
| WOS关键词 | BARR-VIRUS TRANSFORMATION ; CRYOPRESERVED LYMPHOCYTES ; EFFICIENT METHOD ; WHOLE-BLOOD ; IMMORTALIZATION ; INDIVIDUALS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000397847200070 |
| 出版者 | JOURNAL OF VISUALIZED EXPERIMENTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373458 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Duan, Ziyuan |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Genet Resources Ctr, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xi,Xu, Chongfeng,Duan, Ziyuan. A simple red blood cell lysis method for the establishment of b lymphoblastoid cell lines[J]. Jove-journal of visualized experiments,2017(119):6. |
| APA | Liu, Xi,Xu, Chongfeng,&Duan, Ziyuan.(2017).A simple red blood cell lysis method for the establishment of b lymphoblastoid cell lines.Jove-journal of visualized experiments(119),6. |
| MLA | Liu, Xi,et al."A simple red blood cell lysis method for the establishment of b lymphoblastoid cell lines".Jove-journal of visualized experiments .119(2017):6. |
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来源：武汉病毒研究所
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