Fast and sensitive quantification of protein sumoylation using a dual luciferase biosensor
文献类型：期刊论文
| 作者 | Zhang, Yuan; Ke, Xianliang; Liu, Yan; Zheng, Zhenhua; Meng, Jin; Wang, Hanzhong |
| 刊名 | Analyst
 |
| 出版日期 | 2017 |
| 卷号 | 142期号:4页码:608-612 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c6an02479c |
| 通讯作者 | Wang, hanzhong(wanghz@wh.iov.cn) |
| 英文摘要 | Herein a convenient strategy was developed to quantify protein sumoylation. the amount of target proteins and sumo proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the sumoylation degree of the target proteins. |
| WOS关键词 | SINGLE-MOLECULE LEVEL ; FIREFLY LUCIFERASE ; SUMO MODIFICATIONS ; UBIQUITIN ; KINETOCHORES ; BINDING ; CHAINS ; UBC9 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000395649100008 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373466 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, Hanzhong |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuan,Ke, Xianliang,Liu, Yan,et al. Fast and sensitive quantification of protein sumoylation using a dual luciferase biosensor[J]. Analyst,2017,142(4):608-612. |
| APA | Zhang, Yuan,Ke, Xianliang,Liu, Yan,Zheng, Zhenhua,Meng, Jin,&Wang, Hanzhong.(2017).Fast and sensitive quantification of protein sumoylation using a dual luciferase biosensor.Analyst,142(4),608-612. |
| MLA | Zhang, Yuan,et al."Fast and sensitive quantification of protein sumoylation using a dual luciferase biosensor".Analyst 142.4(2017):608-612. |
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来源：武汉病毒研究所
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