Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression
文献类型：期刊论文
| 作者 | Hao, Sujuan1,2; Zhang, Junmei1,2; Chen, Zhen1; Xu, Huanzhou1,2; Wang, Hanzhong1; Guan, Wuxiang1,3 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 91期号:3页码:16 |
| 关键词 | Hbov Alternative polyadenylation Capsid protein expression Parvovirus |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.02026-16 |
| 通讯作者 | Guan, wuxiang(guanwx@wh.iov.cn) |
| 英文摘要 | Alternative processing of human bocavirus (hbov) p5 promoter-transcribed rna is critical for generating the structural and nonstructural protein-encoding mrna transcripts. the regulatory mechanism by which hbov rna transcripts are polyadenylated at proximal [(pa)p] or distal [(pa)d] polyadenylation sites is still unclear. we constructed a recombinant hbov infectious clone to study the alternative polyadenylation regulation of hbov. surprisingly, in addition to the reported distal polyadenylation site, (pa)d, a novel distal polyadenylation site, (pa)d2, which is located in the right-end hairpin (reh), was identified during infectious clone transfection or recombinant virus infection. (pa)d2 does not contain typical hexanucleotide polyadenylation signal, upstream elements (use), or downstream elements (dse) according to sequence analysis. further study showed that hbov nonstructural protein ns1, reh, and cis elements of (pa) d were necessary and sufficient for efficient polyadenylation at (pa)d2. the distance and sequences between (pa)d and (pa)d2 also played a key role in the regulation of polyadenylation at (pa)d2. finally, we demonstrated that efficient polyadenylation at (pa)d2 resulted in increased hbov capsid mrna transcripts and protein translation. thus, our study revealed that all the bocaviruses have distal poly(a) signals on the right-end palindromic terminus, and alternative polyadenylation at the hbov 3' end regulates its capsid expression. importance the distal polyadenylation site, (pa)d, of hbov is located about 400 nucleotides (nt) from the right-end palindromic terminus, which is different from those of bovine parvovirus (bpv) and canine minute virus (mvc) in the same genus whose distal polyadenylation is located in the right-end stem-loop structure. a novel polyadenylation site, (pa)d2, was identified in the right-end hairpin of hbov during infectious clone transfection or recombinant virus infection. sequence analysis showed that (pa)d2 does not contain typical polyadenylation signals, and the last 42 nt form a stem-loop structure which is almost identical to that of mvc. further study showed that ns1, reh, and cis elements of (pa) d are required for efficient polyadenylation at (pa)d2. polyadenylation at (pa)d2 enhances capsid expression. our study demonstrates alternative polyadenylation at the 3' end of hbov and suggests an additional mechanism by which capsid expression is regulated. |
| WOS关键词 | RESPIRATORY-TRACT INFECTIONS ; PRECURSOR MESSENGER-RNA ; HUMAN PARVOVIRUS B19 ; INTERNAL POLYADENYLATION ; ACUTE GASTROENTERITIS ; MINUTE VIRUS ; NONSTRUCTURAL PROTEINS ; CHILDREN ; GENOME ; CELLS |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000393194600026 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373470 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Guan, Wuxiang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Hubei Collaborat Innovat Ctr Ind Fermentat, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Sujuan,Zhang, Junmei,Chen, Zhen,et al. Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression[J]. Journal of virology,2017,91(3):16. |
| APA | Hao, Sujuan,Zhang, Junmei,Chen, Zhen,Xu, Huanzhou,Wang, Hanzhong,&Guan, Wuxiang.(2017).Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression.Journal of virology,91(3),16. |
| MLA | Hao, Sujuan,et al."Alternative polyadenylation of human bocavirus at its 3 ' end is regulated by multiple elements and affects capsid expression".Journal of virology 91.3(2017):16. |
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来源：武汉病毒研究所
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