A new strategy for full-length ebola virus glycoprotein expression in e.coli
文献类型：期刊论文
| 作者 | Zai, Junjie1,2; Yi, Yinhua1,2; Xia, Han1; Zhang, Bo3; Yuan, Zhiming1 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 31期号:6页码:500-508 |
| 关键词 | Ebola virus (ebov) Glycoprotein (gp) E.coli Prokaryotic expression |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-016-3845-y |
| 通讯作者 | Yuan, zhiming(yzm@wh.iov.cn) |
| 英文摘要 | Ebola virus (ebov) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. glycoprotein (gp) is the only envelope protein of ebov, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. however, the lack of expression of full-length gp in escherichia coli hinders the further study of its function in viral pathogenesis. in this study, the vp40 gene was fused to the full-length gp gene and cloned into a prokaryotic expression vector. we showed that the vp40-gp and gp-vp40 fusion proteins could be expressed in e.coli at 16 degrees c. in addition, it was shown that the position of vp40 in the fusion proteins affected the yields of the fusion proteins, with a higher level of production of the fusion protein when vp40 was upstream of gp compared to when it was downstream. the results provide a strategy for the expression of a large quantity of ebov full-length gp, which is of importance for further analyzing the relationship between the structure and function of gp and developing an antibody for the treatment of ebov infection. |
| WOS关键词 | PROTECTS NONHUMAN-PRIMATES ; INSECT CELLS ; GUINEA-PIGS ; PARTICLES ; VACCINE ; GP ; VP40 ; ORGANIZATION ; ANTIBODIES ; CHALLENGE |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000393205300006 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373472 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Ctr Emerging Infect Dis, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zai, Junjie,Yi, Yinhua,Xia, Han,et al. A new strategy for full-length ebola virus glycoprotein expression in e.coli[J]. Virologica sinica,2016,31(6):500-508. |
| APA | Zai, Junjie,Yi, Yinhua,Xia, Han,Zhang, Bo,&Yuan, Zhiming.(2016).A new strategy for full-length ebola virus glycoprotein expression in e.coli.Virologica sinica,31(6),500-508. |
| MLA | Zai, Junjie,et al."A new strategy for full-length ebola virus glycoprotein expression in e.coli".Virologica sinica 31.6(2016):500-508. |
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来源：武汉病毒研究所
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