The role of viral protein ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex
文献类型：期刊论文
| 作者 | Mu, Jingfang1,2; Zhang, Yongli1,2; Hu, Yangyang1,2; Hu, Xue1; Zhou, Yuan1; Chen, Xinwen1; Wang, Yun1 |
| 刊名 | Virologica sinica
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 31期号:6页码:480-489 |
| 关键词 | Autographa californica multiple nucleopolyhedrovirus (acmnpv) Ac34 Arp2/3 complex Nuclear relocation |
| ISSN号 | 1674-0769 |
| DOI | 10.1007/s12250-016-3912-4 |
| 通讯作者 | Chen, xinwen(chenxw@wh.iov.cn) ; Wang, yun(wangyun@wh.iov.cn) |
| 英文摘要 | The actin nucleator actin-related protein complex (arp2/3) is composed of seven subunits: arp2, arp3, p40/arpc1 (p40), p34/arpc2 (p34), p21/arpc3 (p21), p20/arpc4 (p20), and p16/arpc5 (p16). arp2/3 plays crucial roles in a variety of cellular activities through regulation of actin polymerization. autographa californica multiple nucleopolyhedrovirus (acmnpv), one of the best-studied alphabaculoviruses, induces arp2/3 nuclear relocation and mediates nuclear actin polymerization to assist in virus replication. we have demonstrated that ac34, a viral late-gene product, induces translocation of the p40 subunit of arp2/3 to the nucleus during acmnpv infection. however, it remains unknown whether ac34 could relocate other arp2/3 subunits to the nucleus. in this study, the effects of the viral protein ac34 on the distribution of these subunits were studied by an immunofluorescence assay. arp2, p34, p21, and p20 cloned from spodoptera frugiperda (sf9) cells showed mainly cytoplasmic localization and were relocated to the nucleus in the presence of ac34. in addition, arp3 was localized in the cytoplasm in both the presence and absence of ac34, and p16 showed whole-cell localization. in contrast to sf9 cells, all subunits of mammalian arp2/3 showed no nuclear relocation in the presence of ac34. co-immunoprecipitation analysis of the interaction between ac34 and arp2/3 subunits revealed that ac34 bound to p40, p34, and p20 of sf9 cells. however, none of the subunits of mammalian arp2/3 interacted with ac34, indicating that protein-protein interaction is essential for ac34 to relocate arp2/3 subunits to the nucleus. |
| WOS关键词 | CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS ; RNA-POLYMERASE-II ; ARP2/3 COMPLEX ; CELLULAR CONTROL ; GENE-EXPRESSION ; CELLS ; WASP ; POLYMERIZATION ; IDENTIFICATION ; TRANSCRIPTION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000393205300004 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373473 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Chen, Xinwen; Wang, Yun |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Jingfang,Zhang, Yongli,Hu, Yangyang,et al. The role of viral protein ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex[J]. Virologica sinica,2016,31(6):480-489. |
| APA | Mu, Jingfang.,Zhang, Yongli.,Hu, Yangyang.,Hu, Xue.,Zhou, Yuan.,...&Wang, Yun.(2016).The role of viral protein ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex.Virologica sinica,31(6),480-489. |
| MLA | Mu, Jingfang,et al."The role of viral protein ac34 in nuclear relocation of subunits of the actin-related protein 2/3 complex".Virologica sinica 31.6(2016):480-489. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。