A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillin-resistant staphylococcus aureus
文献类型：期刊论文
| 作者 | Yang, Hang; Zhang, Huaidong; Wang, Jing; Yu, Junping; Wei, Hongping |
| 刊名 | Scientific reports
 |
| 出版日期 | 2017-01-09 |
| 卷号 | 7页码:13 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/srep40182 |
| 通讯作者 | Wei, hongping(hpwei@wh.iov.cn) |
| 英文摘要 | Methicillin-resistant staphylococcus aureus (mrsa) is one of the most threatening pathogens due to its multi-drug resistance (mdr) and strong biofilm-forming capacity. here, we described the screening of a novel chimeolysin (clyf) that was active against planktonic and biofilm mrsa. biochemical tests showed that clyf was active against all s. aureus clinical isolates tested under planktonic and biofilm conditions. structure analysis revealed that clyf has an enhanced thermostability and ph tolerance than its parental lysin pc by forming a hydrophobic cleft in the catalytic domain and an ig-like structure in the cell-wall binding domain. a single intraperitoneally or topically administration of clyf showed good mrsa removing efficacy in mouse models of bacteremia and burn wound infection, respectively. our data collectively demonstrated that clyf has good bactericidal activity against planktonic and biofilm mrsa both in vitro and in vivo, and therefore represents a useful antibacterial to combat mdr s. aureus. |
| WOS关键词 | WALL BINDING DOMAINS ; BROAD LYTIC ACTIVITY ; BACTERIOPHAGE ENDOLYSIN ; IN-VITRO ; ANTIMICROBIAL ACTIVITY ; BACILLUS-ANTHRACIS ; CRYSTAL-STRUCTURE ; ANTI-INFECTIVES ; PROTEIN P128 ; ENZYME |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000391376500001 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2373482 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wei, Hongping |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Ctr Emerging Infect Dis, Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Hang,Zhang, Huaidong,Wang, Jing,et al. A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillin-resistant staphylococcus aureus[J]. Scientific reports,2017,7:13. |
| APA | Yang, Hang,Zhang, Huaidong,Wang, Jing,Yu, Junping,&Wei, Hongping.(2017).A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillin-resistant staphylococcus aureus.Scientific reports,7,13. |
| MLA | Yang, Hang,et al."A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillin-resistant staphylococcus aureus".Scientific reports 7(2017):13. |
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来源：武汉病毒研究所
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